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ULB/5 October 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{ULB_Header_2}} "Biobricks construction" *PCR to obtain linearised pSB1C3, using linearised pSB1C3 as template, with the protocol from iGEM parts registry. Didn't work. *PCR on ParD and...) |
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| - | "Biobricks construction | + | "Biobricks construction |
*PCR to obtain linearised pSB1C3, using linearised pSB1C3 as template, with the protocol from iGEM parts registry. Didn't work. | *PCR to obtain linearised pSB1C3, using linearised pSB1C3 as template, with the protocol from iGEM parts registry. Didn't work. | ||
*PCR on ParD and ParE to have those genes outside of a plasmid (as PCR products). Worked. We used a plasmid containing both genes as template. | *PCR on ParD and ParE to have those genes outside of a plasmid (as PCR products). Worked. We used a plasmid containing both genes as template. | ||
*PCR on FLP, using the sequence we got synthesized as backbone, to add RCF 10 prefix and suffix to the gene. Didn't work. | *PCR on FLP, using the sequence we got synthesized as backbone, to add RCF 10 prefix and suffix to the gene. Didn't work. | ||
| - | "Module hydrogen | + | "Module hydrogen |
| + | * We obtained bacteria strains with two deleted genes: one strain that lacked LdhA and FocA and the other one was missing LdhA and PPC. | ||
Revision as of 00:04, 28 October 2010
"Biobricks construction
- PCR to obtain linearised pSB1C3, using linearised pSB1C3 as template, with the protocol from iGEM parts registry. Didn't work.
- PCR on ParD and ParE to have those genes outside of a plasmid (as PCR products). Worked. We used a plasmid containing both genes as template.
- PCR on FLP, using the sequence we got synthesized as backbone, to add RCF 10 prefix and suffix to the gene. Didn't work.
"Module hydrogen
- We obtained bacteria strains with two deleted genes: one strain that lacked LdhA and FocA and the other one was missing LdhA and PPC.
