"
ULB/31 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{ULB_Header_2}} '''Quorum addiction module''' *Verification by PCR of the ligations RBS1+LuxR, RBS0.3+ParD and RBS1+ParD. All of those failed.) |
|||
| Line 2: | Line 2: | ||
'''Quorum addiction module''' | '''Quorum addiction module''' | ||
*Verification by PCR of the ligations RBS1+LuxR, RBS0.3+ParD and RBS1+ParD. All of those failed. | *Verification by PCR of the ligations RBS1+LuxR, RBS0.3+ParD and RBS1+ParD. All of those failed. | ||
| + | |||
| + | '''Module hydrogen''' | ||
| + | * We continue the purification process for the deletion candidate | ||
| + | * We continue to try to obtain other deletion candidates | ||
| + | * We did not manage to ligate tdcE with the RBS. We started again the maxipreparation of the RBS | ||
| + | |||
| + | '''Module homologous recombination''' | ||
| + | * The cm resistance cassette was not inserted properly into the pSB1C3, we started again | ||
| + | * We digested gam and bet to insert them with a RBS | ||
| + | |||
| + | '''Module heavy metals detection''' | ||
| + | * We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper, we let the bacteria grow | ||
| + | |||
| + | '''Other''' | ||
| + | * We try again to obtain more pSB1C3 | ||
Latest revision as of 03:43, 28 October 2010
Quorum addiction module
- Verification by PCR of the ligations RBS1+LuxR, RBS0.3+ParD and RBS1+ParD. All of those failed.
Module hydrogen
- We continue the purification process for the deletion candidate
- We continue to try to obtain other deletion candidates
- We did not manage to ligate tdcE with the RBS. We started again the maxipreparation of the RBS
Module homologous recombination
- The cm resistance cassette was not inserted properly into the pSB1C3, we started again
- We digested gam and bet to insert them with a RBS
Module heavy metals detection
- We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper, we let the bacteria grow
Other
- We try again to obtain more pSB1C3
