ULB/30 August 2010

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Quorum addiction module

  • PCR to verify the ligations/electroporations of the 27/08.
  • Re-ligations of the ones who failed.

Module hydrogen

  • We continue the purification process for the deletion candidate
  • We continue to try to obtain other deletion candidates
  • We try again to ligate tdcE with the RBS

Module homologous recombination

  • The cm resistance cassette was not inserted properly into the pSB1C3
  • We obtained the gene gam and bet by PCR

Module heavy metals detection

  • We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow. The bacteria did not turn orange. We concluded that the gene of the carotene was a fail.
  • We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper

Other

  • We try again to obtain more pSB1C3