ULB/30 August 2010

From 2010.igem.org

(Difference between revisions)
(New page: {{ULB_Header_2}} '''Quorum addiction module''' *PCR to verify the ligations/electroporations of the 27/08. *Re-ligations of the ones who failed.)
 
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*PCR to verify the ligations/electroporations of the 27/08.
*PCR to verify the ligations/electroporations of the 27/08.
*Re-ligations of the ones who failed.
*Re-ligations of the ones who failed.
 +
 +
'''Module hydrogen'''
 +
* We continue the purification process for the deletion candidate
 +
* We continue to try to obtain other deletion candidates
 +
* We try again to ligate tdcE with the RBS
 +
 +
'''Module homologous recombination'''
 +
* The cm resistance cassette was not inserted properly into the pSB1C3
 +
* We obtained the gene gam and bet by PCR
 +
 +
'''Module heavy metals detection'''
 +
* We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow. The bacteria did not turn orange. We concluded that the gene of the carotene was a fail.
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* We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper
 +
 +
'''Other'''
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* We try again to obtain more pSB1C3

Latest revision as of 03:33, 28 October 2010

	
		

	
	
		
		
	

Quorum addiction module

  • PCR to verify the ligations/electroporations of the 27/08.
  • Re-ligations of the ones who failed.

Module hydrogen

  • We continue the purification process for the deletion candidate
  • We continue to try to obtain other deletion candidates
  • We try again to ligate tdcE with the RBS

Module homologous recombination

  • The cm resistance cassette was not inserted properly into the pSB1C3
  • We obtained the gene gam and bet by PCR

Module heavy metals detection

  • We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow. The bacteria did not turn orange. We concluded that the gene of the carotene was a fail.
  • We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper

Other

  • We try again to obtain more pSB1C3