ULB/30 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{ULB_Header_2}} '''Quorum addiction module''' *PCR to verify the ligations/electroporations of the 27/08. *Re-ligations of the ones who failed.) |
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*PCR to verify the ligations/electroporations of the 27/08. | *PCR to verify the ligations/electroporations of the 27/08. | ||
*Re-ligations of the ones who failed. | *Re-ligations of the ones who failed. | ||
+ | |||
+ | '''Module hydrogen''' | ||
+ | * We continue the purification process for the deletion candidate | ||
+ | * We continue to try to obtain other deletion candidates | ||
+ | * We try again to ligate tdcE with the RBS | ||
+ | |||
+ | '''Module homologous recombination''' | ||
+ | * The cm resistance cassette was not inserted properly into the pSB1C3 | ||
+ | * We obtained the gene gam and bet by PCR | ||
+ | |||
+ | '''Module heavy metals detection''' | ||
+ | * We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow. The bacteria did not turn orange. We concluded that the gene of the carotene was a fail. | ||
+ | * We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper | ||
+ | |||
+ | '''Other''' | ||
+ | * We try again to obtain more pSB1C3 |
Latest revision as of 03:33, 28 October 2010
Quorum addiction module
- PCR to verify the ligations/electroporations of the 27/08.
- Re-ligations of the ones who failed.
Module hydrogen
- We continue the purification process for the deletion candidate
- We continue to try to obtain other deletion candidates
- We try again to ligate tdcE with the RBS
Module homologous recombination
- The cm resistance cassette was not inserted properly into the pSB1C3
- We obtained the gene gam and bet by PCR
Module heavy metals detection
- We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow. The bacteria did not turn orange. We concluded that the gene of the carotene was a fail.
- We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper
Other
- We try again to obtain more pSB1C3