ULB/26 August 2010

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Quorum addiction module

  • The minipreps done on the 26 contained some genomic DNA, so we purified them on gel. Sadly, the purification didn't work, all the DNA was lost.
  • Made the minipreps again.
  • First ligations of the various pieces to build the two plasmids.

Module hydrogen

  • The tests on the other deletion candidates are once again all negative
  • We continue the purification process for the deletion candidate
  • We continue to try to obtain other deletion candidates

Module homologous recombination

  • We inserted the cm and kan resistance cassette into the pSB1C3

Module heavy metals detection

  • We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow
  • We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow

Other

  • We did a PCR on pSB1C3 in order to have some more