ULB/26 August 2010

From 2010.igem.org

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'''Module hydrogen'''
'''Module hydrogen'''
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* The test on the other deletion candidate are once again all negative
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* The tests on the other deletion candidates are once again all negative
* We continue the purification process for the deletion candidate
* We continue the purification process for the deletion candidate
* We continue to try to obtain other deletion candidates
* We continue to try to obtain other deletion candidates

Latest revision as of 03:14, 28 October 2010

Quorum addiction module

  • The minipreps done on the 26 contained some genomic DNA, so we purified them on gel. Sadly, the purification didn't work, all the DNA was lost.
  • Made the minipreps again.
  • First ligations of the various pieces to build the two plasmids.

Module hydrogen

  • The tests on the other deletion candidates are once again all negative
  • We continue the purification process for the deletion candidate
  • We continue to try to obtain other deletion candidates

Module homologous recombination

  • We inserted the cm and kan resistance cassette into the pSB1C3

Module heavy metals detection

  • We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow
  • We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow

Other

  • We did a PCR on pSB1C3 in order to have some more
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