Toronto/9 September 2010

Revision as of 23:20, 26 October 2010 by Elena P (Talk | contribs)

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Transformations worked in:

E4 BL21 → took 3 samples

DH5a → took 1 sample

Lawns in all DH5a cells→ re-streaked using 4-quadrant technique

-Make more Amp plates tomorrow

Tomorrow:

-Make Amp plates

-Transform bacteria with ligated DNA to the BioBrick vector

-See if the re-streaked plates worked. If not, transform again with E3-E5b (BL21 seems to work better)

-Continue miniprep/digest/gel with today’s cultures growing overnight.