Toronto/22 October 2010


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Innoculate 0.5 ml overnight culture (x2) into 500 ml flasks containing 200ml LB broth, grow to O.D.600=1.0 representing 109 cells per ml.

Pour LB plates and allow to cool. Invert and label plates with exposure, time and dilution factors.

Innoculate 0.5 ml of culture (x2) into two 100 ml flasks containing 20 ml MM2 media. Add 30 ml MM2 media, one containing Catechol at 50mM concentration and incubate with shaking at 37C for six hours. Remove aliquots and plate dilutions every two hours and place plates in 37c incubator overnight.