Toronto/13 August 2010
From 2010.igem.org
-Plates Blank?!?!
-PET vector miniprepped (50uL, ??? mg/uL)
-PaD100 vector miniprepped (50uL, 172 ug/uL)
Digestion of PET vector for E1-->E5b insertion (Prepared through PCR)
Taking: 2uL of pET circular DNA plasmid
2.5uL of 10x NEB Buffer 4
0.25 enzymes BamHI and NdeI each
2uL of ddH2O