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Toronto/13 August 2010
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Elena P (Talk | contribs)
(New page: -Plates Blank?!?! -PET vector miniprepped (50uL, ??? mg/uL) -PaD100 vector miniprepped (50uL, 172 ug/uL) Digestion of PET vector for E1-->E5b insertion (Prepared through PCR) Taking:...)
(New page: -Plates Blank?!?! -PET vector miniprepped (50uL, ??? mg/uL) -PaD100 vector miniprepped (50uL, 172 ug/uL) Digestion of PET vector for E1-->E5b insertion (Prepared through PCR) Taking:...)
Latest revision as of 22:47, 26 October 2010
-Plates Blank?!?!
-PET vector miniprepped (50uL, ??? mg/uL)
-PaD100 vector miniprepped (50uL, 172 ug/uL)
Digestion of PET vector for E1-->E5b insertion (Prepared through PCR)
Taking: 2uL of pET circular DNA plasmid
2.5uL of 10x NEB Buffer 4
0.25 enzymes BamHI and NdeI each
2uL of ddH2O
