TeamNewcastleNanoDrop Spectrophotometer

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=NanoDrop Spectrophotometer=
[[Image:Newcastle_nanodrop_2.jpeg|thumb|350px]]
[[Image:Newcastle_nanodrop_2.jpeg|thumb|350px]]
[[Image:Newcastle_nanodrop_1.jpeg|thumb|350px]]
[[Image:Newcastle_nanodrop_1.jpeg|thumb|350px]]
[[Image:Newcastle_nanodrop_3.jpeg|thumb|350px]]
[[Image:Newcastle_nanodrop_3.jpeg|thumb|350px]]
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Nanodrop can be used to measure the DNA, RNA and protein
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Nanodrop uses absorbance to measure the concentration and purity of DNA, RNA and protein.
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The ideal concentration of DNA is 150 ng/ml.
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Measure the concentration and purity of extracted DNA using absorbance (using the automated nanodrop machine!)
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==Materials required==
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* Pipettes
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* Nanodrop machine
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* Appropriate blanking solution
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* Appropriate samples
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The ideal concentration of DNA is 150 ng/ml!
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==Procedures==
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== Method: ==
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# Log onto computer and select Nanodrop program from the desktop (ND 1000)
# Log onto computer and select Nanodrop program from the desktop (ND 1000)
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# To clean Nanodrop machine wipe pedestal and top and add 3 µl of water to nib of pedestal. Press blank.
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# Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.[[Image:drop3.jpg|150px]]  [[Image:drop.jpg|150px]]  [[Image:drop2.jpg|150px]]
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# Wipe the water off, to initialise/equalizen the equipment add 3 μl of the elution buffer [EB] used in the sample and press blank. Set to DNA-50 for DNA.  
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# After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (example: Miniprep samples should be equalized with EB buffer).
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# Use DNA-50 for DNA samples.  
# Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.  
# Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.  
# If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
# If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
# After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.
# After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.
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'''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]'''
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{{Team:Newcastle/footer}}

Latest revision as of 10:26, 13 August 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

NanoDrop Spectrophotometer

Newcastle nanodrop 2.jpeg
Newcastle nanodrop 1.jpeg
Newcastle nanodrop 3.jpeg

Nanodrop uses absorbance to measure the concentration and purity of DNA, RNA and protein. The ideal concentration of DNA is 150 ng/ml.

Materials required

  • Pipettes
  • Nanodrop machine
  • Appropriate blanking solution
  • Appropriate samples

Procedures

  1. Log onto computer and select Nanodrop program from the desktop (ND 1000)
  2. Wipe the pedestal and top of the Nanodrop machine with a tissue. Place 3 µl of water to nib of pedestal and press blank to clean.Drop3.jpg Drop.jpg Drop2.jpg
  3. After blanking, wipe the water off and equalize the Nanodrop using 3 μl of the appropriate buffer used to resuspend the sample. (example: Miniprep samples should be equalized with EB buffer).
  4. Use DNA-50 for DNA samples.
  5. Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
  6. If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
  7. After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.


Go back to our Protocol List

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