Team:Yale/Our Project/Notebook/Week 9

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<li> The PCR products were then run on a 1.0% agarose gel at 90 V vs. a 1 kb ladder.</li>
<li> The PCR products were then run on a 1.0% agarose gel at 90 V vs. a 1 kb ladder.</li>
Loaded ladder at the far left and samples 1 through 14 from left to right in order.  Only sample 6 is even close the size range of the desired ligation product (8.3 kb) and even it is still at least a kb too small to be the right construct. <br/>
Loaded ladder at the far left and samples 1 through 14 from left to right in order.  Only sample 6 is even close the size range of the desired ligation product (8.3 kb) and even it is still at least a kb too small to be the right construct. <br/>
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<img src="https://static.igem.org/mediawiki/2010/f/f9/Yale-colony-pcr2.jpg" />
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<i> This day's labwork is also recorded on pages 85 and 86 of the hard copy lab notebook. </i>
<i> This day's labwork is also recorded on pages 85 and 86 of the hard copy lab notebook. </i>
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Latest revision as of 03:26, 28 October 2010

iGEM Yale

lab notebook: week 9

  • Monday 8/2--Analysis of second ligation transformants
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