Team:Yale/Our Project/Notebook/Week 8

From 2010.igem.org

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<li> Reran the double digest of 7/27, but repeating the gel was made unnecessary by the arrival of sequencing data confirming the successful ligation of phsABC into B0015. </li>
<li> Reran the double digest of 7/27, but repeating the gel was made unnecessary by the arrival of sequencing data confirming the successful ligation of phsABC into B0015. </li>
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<a id="link" href="javascript:ReverseDisplay('sequence data')">See/hide sequence data</a>
 
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<!------------- atgc ------------->
 
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>sequence of desired phsABC/B0015 construct
 
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<!------------- atgc ------------->
 
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<h4>Beginning of second ligation efforts </h4>
<h4>Beginning of second ligation efforts </h4>

Revision as of 03:23, 28 October 2010

iGEM Yale

lab notebook: week 8 (7/26-8/1)

  • Monday 7/26--Efforts to confirm that ligation of phsABC and B0015 actually occurred.
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  • Tuesday 7/27--Diagnostic double digest of likely ligations
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  • Wednesday 7/28--Sequencing confirms ligation success!
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  • Thursday 7/29--First attempt at Q04121 ligation
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  • Friday 7/30--No colonies visible from 7/29 transformation, so returned plates to incubator.
  • Saturday 7/31--Plates from ligation transformation showed growth.
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