Team:Yale/Our Project/Notebook/Week 7

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iGEM Yale

lab notebook: week 7 (7/19-7/25)

  • Monday 7/19--Investigation of how pre-ligation processing affects ligation results
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  • Tuesday 7/20--Self-ligation of single phsABC digestions and serial digestion of ligation components
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  • Wednesday 7/21--analysis of self-ligation, tranformation of Biobrick Q04121, and set up of ligation attempt #6
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  • Thursday 7/22--Ligation Transformations
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  • Friday 7/23--Results of ligation transformations
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  • Saturday 7/24--Gel of colony PCR suggest a successful ligation of phsABC into B0015!
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  • Sunday 7/25--Inoculate cultures of likely ligations successes for miniprep on 7/26
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