Team:Yale/Our Project/Notebook/Week 7

From 2010.igem.org

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<b> Inoculation of liquid cultures </b> <br/>
<b> Inoculation of liquid cultures </b> <br/>
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<li> Inoculated liquid ampicillin in LB cultures from the colonies shown to contain successful ligations by the 9/25 gel of colony PCR.  Also inoculated a culture of Q04121 for overnight growth, adding kanamycin to a concentration of 50 ng/uL. </li>
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<i>This day's labwork is also recorded on page 81 of the hard copy lab notebook </i>
<i>This day's labwork is also recorded on page 81 of the hard copy lab notebook </i>
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Revision as of 00:50, 28 October 2010

iGEM Yale

lab notebook: week 7 (7/19-7/25)

  • Monday 7/19--Investigation of how pre-ligation processing affects ligation results
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  • Tuesday 7/20--Self-ligation of single phsABC digestions and serial digestion of ligation components
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  • Wednesday 7/21--analysis of self-ligation, tranformation of Biobrick Q04121, and set up of ligation attempt #6
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  • Thursday 7/22--Ligation Transformations
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  • Friday 7/23--Results of ligation transformations
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  • Saturday 7/24--Gel of colony PCR suggest a successful ligation of phsABC into B0015!
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  • Sunday 7/25--Inoculate cultures of likely ligations successes for miniprep on 7/26
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