Team:Yale/Our Project/Notebook/Week 5

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iGEM Yale

lab notebook: week 5 (7/5 -7/11)

  • Monday 7/5--Colony PCR of Ligation Attempt #2 Transformants
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  • Tuesday 7/6--Analysis of Results of Ligation Attempts 1 & 2
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  • Wednesday 7/7--Triple ligation attempt--promoter + thiosulfate reductase + terminator
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  • Thursday 7/8--Further ligation efforts
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  • Friday 7/9--Ligation attempt #4 to join phsABC and B0015 terminator.
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  • Saturday 7/10--Transformants from ligation attempts
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  • Sunday 7/11--Prep for copper growth assays of transformants
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