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Team:Yale/Our Project/Notebook/Week 5
From 2010.igem.org
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| - | Monday | + | Monday 7/5--Colony PCR of Ligation Attempt #2 Transformants |
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| - | + | <h4> Colony PCR</h4> | |
| + | <ul> | ||
| + | <li>Ran colony PCR of colonies 1B-9B from ligation attempt #2 according to the same protocol as used on <a href="https://2010.igem.org/Team:Yale/Our_Project/Notebook/Week_4"> 7/1</a>.</li> | ||
| + | <li>Then ran a 1.0% agarose gel of the nine PCR reaction solutions against a 1 kb ladder, but the resulting gel failed to show any evidence of either insert or vector, so will have to rely on sequencing and digests of miniprepped plasmids.</li> | ||
| + | Gel of colony PCR from ligation attempt #2 Lane 1: 1 kb ladder, Lanes 3-11:PCR products from colonies 1B-9B, in order<br/> | ||
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| + | <li>Miniprepped liquid cultures grown from colonies of first ligation attempt (colonies 1A-4A) and second ligation attempt (colonies 1B-9B) according to <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols"> standard miniprep protocol.</a> </li> | ||
| + | </ul> | ||
| + | <i>This day's activities are also recorded on page 47 and 48 of the hard copy lab notebook. </i> | ||
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Revision as of 12:31, 27 October 2010
our project
