Team:Yale/Our Project/Notebook/Week 4

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<li id="nb"><a href="http://2010.igem.org/Team:Yale/Our Project/Notebook/Week 7">week 7</a></li>
<li id="nb"><a href="http://2010.igem.org/Team:Yale/Our Project/Notebook/Week 7">week 7</a></li>
<li id="nb"><a href="http://2010.igem.org/Team:Yale/Our Project/Notebook/Week 8">week 8</a></li>
<li id="nb"><a href="http://2010.igem.org/Team:Yale/Our Project/Notebook/Week 8">week 8</a></li>
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<li id="nb"><a href="http://2010.igem.org/Team:Yale/Our Project/Notebook/Week 9">week 9</a></li>
<li><a href="http://2010.igem.org/Team:Yale/Our Project/Protocols">protocols</a></li>
<li><a href="http://2010.igem.org/Team:Yale/Our Project/Protocols">protocols</a></li>
<li><a href="http://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>
<li><a href="http://2010.igem.org/Team:Yale/Our Project/Safety">safety</a></li>

Latest revision as of 02:50, 28 October 2010

iGEM Yale

lab notebook: week 4 (6/28 -7/4)

  • Monday 6/28--PCR amplification of thiosulfate reductase operon phsABC
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  • Tuesday 6/29--Gel extraction of PCR amplified phs, TSI agar slant assay for hydrogen sulfide production, & pre-ligation double digestion
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  • Wednesday 6/30--Troubleshooting PCR amplification of thiosulfate reductase gene & continued work toward ligation
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  • Thursday 7/1--Smelly bacteria (yay!) & ongoing ligation efforts
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  • Friday 7/2--Confirmation of hydrogen sulfide production plus more ligation work
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  • Saturday 7/3--More attempts to put a terminator on thiosulfate reductase and a promoter on lacI
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  • Sunday 7/4--Ligation attempt #2 transformants
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