Team:Yale/Our Project/Notebook/Week 4

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<li>Slant inoculated with the LE392 transformants containing pSB74 showed a mass of black precipitate near the surface, definitively confirming the production of hydrogen sulfide (!), the smell of which was by now very strong. The DH5alpha transformants did not exhibit the same H<sub>2</sub>S odor.</li>
<li>Slant inoculated with the LE392 transformants containing pSB74 showed a mass of black precipitate near the surface, definitively confirming the production of hydrogen sulfide (!), the smell of which was by now very strong. The DH5alpha transformants did not exhibit the same H<sub>2</sub>S odor.</li>
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       blurry (apologies!) image of LE392 slant showing FeS precipitate(right) next to un-inoculated slant (left)
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<img src="http://2010.igem.org/wiki/images/3/38/Yale-tsi-agar.png" />
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       <div id="caption>blurry (apologies!) image of LE392 slant showing FeS precipitate(right) next to un-inoculated slant (left)</div>
        
        
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Revision as of 21:28, 27 October 2010

iGEM Yale

lab notebook: week 4 (6/28 -7/4)

  • Monday 6/28--PCR amplification of thiosulfate reductase operon phsABC
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  • Tuesday 6/29--Gel extraction of PCR amplified phs, TSI agar slant assay for hydrogen sulfide production, & pre-ligation double digestion
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  • Wednesday 6/30--Troubleshooting PCR amplification of thiosulfate reductase gene & continued work toward ligation
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  • Thursday 7/1--Smelly bacteria (yay!) & ongoing ligation efforts
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  • Friday 7/2--Confirmation of hydrogen sulfide production plus more ligation work
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