Team:Yale/Our Project/Notebook/Week 4

From 2010.igem.org

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Revision as of 11:57, 27 October 2010

iGEM Yale

lab notebook: week 4 (6/28 -7/4)

  • Monday 6/28--PCR amplification of thiosulfate reductase operon phsABC
  • See more/less
  • Tuesday 6/29--Gel extraction of PCR amplified phs, TSI agar slant assay for hydrogen sulfide production, & pre-ligation double digestion
  • See more/less
  • Wednesday 6/30--Troubleshooting PCR amplification of thiosulfate reductase gene & continued work toward ligation
  • See more/less
  • Thursday 7/1--Smelly bacteria (yay!) & ongoing ligation efforts
  • See more/less
  • Friday 7/2--Confirmation of hydrogen sulfide production plus more ligation work
  • See more/less
  • Saturday 7/3--More attempts to put a terminator on thiosulfate reductase and a promoter on lacI
  • See more/less
  • Sunday 7/4--Ligation attempt #2 transformants
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