Team:Yale/Our Project/Notebook/Week 4

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<h4> TSI agar assay </h4>
<h4> TSI agar assay </h4>
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<li>LE392 and DH5alpha cultures in TSI Agar showed some growth. No black color change was visible, but both slants had a rotten egg scent, so suspected generation of H2S at concentrations too low to visibly trigger color change yet.</li><br/>
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<li>LE392 and DH5alpha cultures in TSI agar showed some growth. No black color change was visible, but both slants had a rotten egg scent, so suspected generation of H<sub>2</sub>S at concentrations too low to visibly trigger color change yet.</li><br/>
</ul>
</ul>
<h4>PCR work</h4>
<h4>PCR work</h4>

Revision as of 11:34, 27 October 2010

iGEM Yale

lab notebook: week 4 (6/28 -7/4)

  • Monday 6/28--PCR amplification of thiosulfate reductase operon phsABC
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  • Tuesday 6/29--Gel extraction of PCR amplified phs, TSI agar slant assay for hydrogen sulfide production, & pre-ligation double digestion
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  • Wednesday 6/30--Troubleshooting PCR amplification of thiosulfate reductase gene & continued work toward ligation
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  • Thursday 7/1--Smelly bacteria (yay!) & ongoing ligation efforts
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  • Fridays short content
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  • Saturday short content
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  • Sunday short content
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