Team:Yale/Our Project/Notebook/Week 3
From 2010.igem.org
(Difference between revisions)
m |
|||
Line 112: | Line 112: | ||
So that can readily determine whether bacteria are producing hydrogen sulfide, created a batch of TSI agar slants according to the following <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar"> recipe </a>, with ampicillin as the added antibiotic and tubes of the medium allowed to solidify on an angle to produce a characteristic slant. If a culture containing thiosulfate reductase is grown in such a slant, it will turn the medium black by precipitating out black iron sulfide, an obvious visual cue of hydrogen sulfide production. <br/> | So that can readily determine whether bacteria are producing hydrogen sulfide, created a batch of TSI agar slants according to the following <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/TSI_agar"> recipe </a>, with ampicillin as the added antibiotic and tubes of the medium allowed to solidify on an angle to produce a characteristic slant. If a culture containing thiosulfate reductase is grown in such a slant, it will turn the medium black by precipitating out black iron sulfide, an obvious visual cue of hydrogen sulfide production. <br/> | ||
<b> Transformation of BBa_P0312</b> <br/> | <b> Transformation of BBa_P0312</b> <br/> | ||
- | After realizing that Biobrick P0312 can be used in place of C0012 and R0011, transformed LE392 with P0312 according to the <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/transformation"> standard transformation protocol </a> and plated on ampicillin LB plates to grow overnight in the incubator at 37˚C. (Because no Amp LB plates were prepared, simply spread the antibiotic topically on a plain LB plate prior to adding transformants.) | + | After realizing that Biobrick P0312 can be used in place of C0012 and R0011, transformed LE392 with P0312 according to the <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/transformation"> standard transformation protocol </a> and plated on ampicillin LB plates to grow overnight in the incubator at 37˚C. (Because no Amp LB plates were prepared, simply spread the antibiotic topically on a plain LB plate prior to adding transformants.)<br/> |
<i> This day's labwork is also recorded on pages 18, 20, and 21 of the hard copy lab notebook. </i> | <i> This day's labwork is also recorded on pages 18, 20, and 21 of the hard copy lab notebook. </i> | ||
<!------------- Wednesday -------------> | <!------------- Wednesday -------------> |
Revision as of 11:07, 27 October 2010
our project
lab notebook: week 3 (6/21-6/27)
- Monday 6/21--Miniprep Biobrick plasmids from overnight culture, but a mix up samples, necessitating a diagnostic digest. See more/less
- Tuesday 6/22--Miniprep redos See more/less
- Wednesday 6/23--Making TSI agar slants & transformation of P0312 See more/less
- Thursday 6/24--primer ordering & IPTG-inducible promoter(P0312) transformants See more/less
- Friday 6/25--P0312 miniprep, pSB74 transform, & pouring plates See more/less