Team:Yale/Our Project/Notebook/Week 3
From 2010.igem.org
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- | <li> Miniprepped the overnight culture of P0312 in LE392 according to the <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/miniprep"> standard microcentrifuge protocol | + | <li> Miniprepped the overnight culture of P0312 in LE392 according to the <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/miniprep"> standard microcentrifuge protocol </a> and found the resulting plasmid solution to have a concentration of 11.9 ng DNA/uL</li> |
<li>Transformed BL21, LE392, and DH5alpha with pSB74 according to this <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/transformation"> standard transformation protocol </a> <br/> and plated on ampicillin LB plates to incubate overnight at 37˚C. </li> | <li>Transformed BL21, LE392, and DH5alpha with pSB74 according to this <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/transformation"> standard transformation protocol </a> <br/> and plated on ampicillin LB plates to incubate overnight at 37˚C. </li> | ||
<li> Prepared 1 liter's worth of ampicillin LB plates and 500 mL of plain LB plates according to this <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/LB_agar"> recipe. </a> The ampicillin 1000x stock solution was made by dissolving 2.0 g of ampicillin in 20.0 mL of water, filter-sterilizing the resultant solution, and storing it in 1 mL aliquots in the freezer. </li? | <li> Prepared 1 liter's worth of ampicillin LB plates and 500 mL of plain LB plates according to this <a href="https://2010.igem.org/Team:Yale/Our_Project/Protocols/LB_agar"> recipe. </a> The ampicillin 1000x stock solution was made by dissolving 2.0 g of ampicillin in 20.0 mL of water, filter-sterilizing the resultant solution, and storing it in 1 mL aliquots in the freezer. </li? |
Revision as of 08:57, 27 October 2010
our project
lab notebook: week 3 (6/21-6/27)
- Monday 6/21--Miniprep Biobrick plasmids from overnight culture, but a mix up samples, necessitating a diagnostic digest. See more/less
- Tuesday 6/22--Miniprep redos See more/less
- Wednesday 6/23--Making TSI agar slants & transformation of P0312 See more/less
- Thursday 6/24--primer ordering & IPTG-inducible promoter(P0312) transformants See more/less
- Friday 6/25--P0312 miniprep, pSB74 transform, & pouring plates See more/less