Team:Yale/Our Project/Notebook/Week 2

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iGEM Yale

lab notebook: week 2 (6/14-6/20)

  • Monday 6/14--Redo of 6/11 assay of bacterial growth within a narrow copper(II) concentrations after analysis of data showed uniformly poor growth.
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  • Tuesday 6/15--Results and analysis of the 6/14 narrow concentration range growth assay as well as planning for the creation of a standard iGEM plasmid bearing the thiosulfate reductase operon (phsABC).
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  • Wednesday 6/16--Checked on spotted cell survival assay, collected MOPS minimal media materials & started making component solutions
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  • Thursday 6/17--more minimal media work and meeting, started BL21 culture
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  • Friday 6/18--Arrival of plasmid pSB74, transformation of Biobricks, & copper growth assays for BL21 strain.
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  • Saturday 6/19--Redo of Biobrick transformations & analysis of BL21 copper growth assay
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  • Sunday 6/20--Observed growth of all transformants from 6/19, so used them and the culture containing pSB74 to inoculate 5 mL liquid cultures in LB with ampicillin. Left to grow overnight on shaker at 37˚C for miniprep the following morning.
    The activities of this day are also recorded on page 16 of the hard copy lab notebook