Team:Yale/Our Project/Notebook/Week 2

From 2010.igem.org

(Difference between revisions)
Line 13: Line 13:
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Methods">methods</a></li>
<li><a href="https://2010.igem.org/Team:Yale/Our Project/Methods">methods</a></li>
<li><b><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">lab notebook</a></b></li>
<li><b><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">lab notebook</a></b></li>
-
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">week 1</a></b></li>
+
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook">week 1</a></li>
-
<li id="nb"><b><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 2">week 2</a></li>
+
<li id="nb"><b><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 2">week 2</a></b></li>
-
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 3">week 3</a></b></li>
+
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 3">week 3</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 4">week 4</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 4">week 4</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 5">week 5</a></li>
<li id="nb"><a href="https://2010.igem.org/Team:Yale/Our Project/Notebook/Week 5">week 5</a></li>

Revision as of 06:38, 13 October 2010

iGEM Yale

lab notebook: week 2

  • Monday 6/14--Primer design, redo of Friday's growth assay
  • Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived
  • Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions
  • Thursday 6/17--more minimal media work and meeting, started BL21 culture
  • Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges. June the 19th--in which it is revealed that there was a transformation-fail Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge
  • Sunday 6/20--in evening inoculate 5 mL liquid cultures