Team:Yale/Our Project/Notebook/Week 2

From 2010.igem.org

(Difference between revisions)
Line 320: Line 320:
<img src="https://static.igem.org/mediawiki/2010/f/f6/Yale-bl21.jpg" />
<img src="https://static.igem.org/mediawiki/2010/f/f6/Yale-bl21.jpg" />
</div>
</div>
-
It appears that BL21 is slightly more sensitive to copper than the other two strains, since 3 mM levels of copper(II) sulfate are enough to almost completely inhibit BL21's growth.
+
It appears that BL21 is slightly more sensitive to copper than the other two strains, since 3 mM levels of copper(II) sulfate are enough to almost completely inhibit BL21's growth. <br/>
<i> The activities of this day are also recorded on page 15 of the hard copy lab notebook </i>
<i> The activities of this day are also recorded on page 15 of the hard copy lab notebook </i>
<!------------- Saturday ------------->
<!------------- Saturday ------------->

Revision as of 23:06, 27 October 2010

iGEM Yale

lab notebook: week 2 (6/14-6/20)

  • Monday 6/14--Redo of 6/11 assay of bacterial growth within a narrow copper(II) concentrations after analysis of data showed uniformly poor growth.
  • See more/less
  • Tuesday 6/15--Results and analysis of the 6/14 narrow concentration range growth assay as well as planning for the creation of a standard iGEM plasmid bearing the thiosulfate reductase operon (phsABC).
  • See more/less
  • Wednesday 6/16--Checked on spotted cell survival assay, collected MOPS minimal media materials & started making component solutions
  • See more/less
  • Thursday 6/17--more minimal media work and meeting, started BL21 culture
  • See more/less
  • Friday 6/18--Arrival of plasmid pSB74, transformation of Biobricks, & copper growth assays for BL21 strain.
  • See more/less
  • Saturday 6/19--Redo of Biobrick transformations & analysis of BL21 copper growth assay
  • See more/less
  • Sunday 6/20--Observed growth of all transformants from 6/19, so used them and the culture containing pSB74 to inoculate 5 mL liquid cultures in LB with ampicillin. Left to grow overnight on shaker at 37˚C for miniprep the following morning.
    The activities of this day are also recorded on page 16 of the hard copy lab notebook