Team:Yale/Our Project/Notebook/Week 2

From 2010.igem.org

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It is unsurprising that all cells should die at 1 M copper sulfate concentrations, but the lack of growth of DH5alpha at 0 M, 900 uM, etc. seems anomalous, given that the cells clearly survived at higher copper levels and may reflect an insufficient amount of culture spotted in those cases. <br/>
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<i>This information is also recorded on page 11 of the hard copy lab notebook. </i> <br/>
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Revision as of 03:51, 27 October 2010

iGEM Yale

lab notebook: week 2 (6/14-6/20)

  • Monday 6/14--Redo of 6/11 assay of bacterial growth within a narrow copper(II) concentrations after analysis of data showed uniformly poor growth.
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  • Tuesday 6/15--Results and analysis of the 6/14 narrow concentration range growth assay as well as planning for the creation of a standard iGEM plasmid bearing the thiosulfate reductase operon (phsABC).
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  • Wednesday 6/16--Checked on spotted cell survival assay, collected MOPS minimal media materials & started making component solutions
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  • Thursday 6/17--more minimal media work and meeting, started BL21 culture
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  • Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges. June the 19th--in which it is revealed that there was a transformation-fail Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge
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  • Sunday 6/20--in evening inoculate 5 mL liquid cultures
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