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Team:Yale/Our Project/Notebook/Week 2
From 2010.igem.org
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<p> | <p> | ||
<ul> | <ul> | ||
| - | <!------------- LAB NOTEBOOK | + | <!------------- LAB NOTEBOOK -------------> |
| - | <li>Monday 6/14--Primer design, redo of Friday's growth assay</li> | + | <li> |
| - | <li>Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived</li> | + | |
| - | <li>Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions</li> | + | Monday 6/14--Primer design, redo of Friday's growth assay |
| - | <li>Thursday 6/17--more minimal media work and meeting, started BL21 culture</li> | + | |
| - | <li>Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges. | + | </li> |
| + | <a id="link" href="#" onclick="toggle_visibility('monday');">See more</a> | ||
| + | <div style="display:none" id="monday"> | ||
| + | |||
| + | <!------------- Monday -------------> | ||
| + | Extra content | ||
| + | <!------------- Monday -------------> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <li> | ||
| + | |||
| + | Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived | ||
| + | |||
| + | </li> | ||
| + | <a id="link" href="#" onclick="toggle_visibility('tuesday');">See more</a> | ||
| + | <div style="display:none" id="tuesday"> | ||
| + | |||
| + | <!------------- Tuesday -------------> | ||
| + | Extra content | ||
| + | <!------------- Tuesday -------------> | ||
| + | </div> | ||
| + | |||
| + | <li> | ||
| + | |||
| + | Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions | ||
| + | |||
| + | </li> | ||
| + | <a id="link" href="#" onclick="toggle_visibility('wednesday');">See more</a> | ||
| + | <div style="display:none" id="wednesday"> | ||
| + | |||
| + | <!------------- Wednesday -------------> | ||
| + | Extra content | ||
| + | <!------------- Wednesday -------------> | ||
| + | </div> | ||
| + | |||
| + | <li> | ||
| + | |||
| + | Thursday 6/17--more minimal media work and meeting, started BL21 culture | ||
| + | |||
| + | </li> | ||
| + | <a id="link" href="#" onclick="toggle_visibility('thursday');">See more</a> | ||
| + | <div style="display:none" id="thursday"> | ||
| + | |||
| + | <!------------- Thursday -------------> | ||
| + | Extra content | ||
| + | <!------------- Thursday -------------> | ||
| + | </div> | ||
| + | <li> | ||
| + | |||
| + | Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges. | ||
June the 19th--in which it is revealed that there was a transformation-fail | June the 19th--in which it is revealed that there was a transformation-fail | ||
| - | Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge</li> | + | Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge |
| - | <li>Sunday 6/20--in evening inoculate 5 mL liquid cultures</li> | + | |
| + | </li> | ||
| + | <a id="link" href="#" onclick="toggle_visibility('friday');">See more</a> | ||
| + | <div style="display:none" id="friday"> | ||
| + | <!------------- Friday -------------> | ||
| + | Extra content | ||
| + | <!------------- Friday -------------> | ||
| + | </div> | ||
| + | |||
| + | <li> | ||
| + | |||
| + | Sunday 6/20--in evening inoculate 5 mL liquid cultures | ||
| + | |||
| + | </li> | ||
| + | <a id="link" href="#" onclick="toggle_visibility('sunday');">See more</a> | ||
| + | <div style="display:none" id="sunday"> | ||
| + | <!------------- Sunday -------------> | ||
| + | Extra content | ||
| + | <!------------- Sunday -------------> | ||
| + | </div> | ||
| + | |||
| + | <!------------- LAB NOTEBOOK -------------> | ||
| - | |||
</ul> | </ul> | ||
</p> | </p> | ||
Revision as of 06:09, 23 October 2010
our project
lab notebook: week 2
- Monday 6/14--Primer design, redo of Friday's growth assay See more
- Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived See more
- Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions See more
- Thursday 6/17--more minimal media work and meeting, started BL21 culture See more
- Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges. June the 19th--in which it is revealed that there was a transformation-fail Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge See more
- Sunday 6/20--in evening inoculate 5 mL liquid cultures See more
