Team:Yale/Our Project/Notebook

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iGEM Yale

lab notebook: week 1 (6/7 - 6/13)

  • Monday 6/7--We successfully defend our project to our sponsors(yay!)
  • Wednesday 6/9--First day in lab!--got set up & started cultures of two E. coli strains
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  • Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran an assay measuring bacterial growth in the presence of copper, trying both strains at three different initial ODs and measuring for four hours
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  • Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--b/c allowed to overgrow?
  • See more/less *Bang S. W., Clark D. S., Keasling J. D. Cadmium, lead, and zinc removal by expression of thiosulfate reductase gene from Salmonella typhimurium in Escherichia coli. Biotechnol. Lett. 2000; 22: 1331–1335,