Team:Yale/Our Project/Notebook

From 2010.igem.org

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<h4> Baseline Wide Concentration Range Copper Growth Assay </h4>
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<h5> Baseline Wide Concentration Range Copper Growth Assay </h5>
Set out to find baseline growth exhibited by the E. coli strains being used when grown in a variety of copper concentrations. This will serve as a basis for comparison once copper tolerance has been engineered. Chose a range from the very, very low (sure to have no effect) to the very, very high (surely lethal).
Set out to find baseline growth exhibited by the E. coli strains being used when grown in a variety of copper concentrations. This will serve as a basis for comparison once copper tolerance has been engineered. Chose a range from the very, very low (sure to have no effect) to the very, very high (surely lethal).
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<h4> Results & Conclusions </h4>
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<h5> Results & Conclusions </h5>
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     <li> Both cells lines exhibit very similar copper tolerances, growing without noticeable effect at concentrations up to 500 uM, experiencing slowed growth at 1 mM, and no growth at 10 mM. </li>
     <li> Both cells lines exhibit very similar copper tolerances, growing without noticeable effect at concentrations up to 500 uM, experiencing slowed growth at 1 mM, and no growth at 10 mM. </li>
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<h4> Further Copper Growth Assays—Narrow Concentration Range </h4>
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<h5> Further Copper Growth Assays—Narrow Concentration Range </h5>
Plan to do more assays of bacterial growth in the presence of copper to determine the exact threshold at which growth is affected.  Will repeat yesterday’s assay with a more targeted concentration range and a longer growth time.  
Plan to do more assays of bacterial growth in the presence of copper to determine the exact threshold at which growth is affected.  Will repeat yesterday’s assay with a more targeted concentration range and a longer growth time.  
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Revision as of 01:44, 27 October 2010

iGEM Yale

lab notebook: week 1 (6/7 - 6/13)

  • Monday 6/7--We successfully defend our project to our sponsors(yay!)
  • Wednesday 6/9 --First day in lab!--got set up & started cultures of two E. coli strains
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  • Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran an assay measuring bacterial growth in the presence of copper, trying both strains at three different initial ODs and measuring for four hours
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  • Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--perhaps because allowed to overgrow prior to assay?
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