Team:Yale/Our Project/Notebook

From 2010.igem.org

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Plan to do more assays of bacterial growth in the presence of copper to determine the exact threshold at which growth is affected.  Will repeat yesterday’s assay (link) with a more targeted concentration range and a longer growth time.  
Plan to do more assays of bacterial growth in the presence of copper to determine the exact threshold at which growth is affected.  Will repeat yesterday’s assay (link) with a more targeted concentration range and a longer growth time.  
<ul>
<ul>
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<li>Once again inoculate liquid cultures of DH5alpha and LE392 in 5 mL LB using bacteria from plates established on 6/9 (link) and put on shaker to grow at 37˚C </li>
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<li>Once again inoculated liquid cultures of DH5alpha and LE392 in 5 mL LB using bacteria from plates established on 6/9 (link) and put on shaker to grow at 37˚C </li>
<li>Let grow for approximately 6 hours, by which point the DH5alpha have reached an OD (measured at 600 nm) of 0.915and the LE392 have reached OD 1.420. </li>
<li>Let grow for approximately 6 hours, by which point the DH5alpha have reached an OD (measured at 600 nm) of 0.915and the LE392 have reached OD 1.420. </li>
<li>Diluted them to the three initial ODs chosen on 6/10 (link) in a variety of copper (II) sulfate concentrations chosen to give more detailed data than the wide concentration range trial. The concentrations chosen were  500 μM, 600 μM, 700 μM, 800 μM, 900 μM, 1 mM, 1.25 mM, 1.5 mM, 2 mM, 2.5 mM, 3 mM, 4 mM </li>
<li>Diluted them to the three initial ODs chosen on 6/10 (link) in a variety of copper (II) sulfate concentrations chosen to give more detailed data than the wide concentration range trial. The concentrations chosen were  500 μM, 600 μM, 700 μM, 800 μM, 900 μM, 1 mM, 1.25 mM, 1.5 mM, 2 mM, 2.5 mM, 3 mM, 4 mM </li>

Revision as of 16:44, 26 October 2010

iGEM Yale

lab notebook: week 1 (6/7 - 6/13)

  • Monday 6/7--We successfully defend our project to our sponsors(yay!)
  • Wednesday 6/9--First day in lab!--got set up & started cultures of two E. coli strains
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  • Thursday 6/10--inoculated liquid cultures in AM, made up different copper sulfate & LB solutions, then ran an assay measuring bacterial growth in the presence of copper, trying both strains at three different initial ODs and measuring for four hours
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  • Friday 6/11--Ran another growth assay with middling concentrations based on results of first, but bacteria failed to grow well--perhaps because allowed to overgrow prior to assay?
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