Team:Yale/Our Project/Methods

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iGEM Yale

experimental methods

Our plasmid is composed of three parts: a promoter and a terminator Biobrick as well as a novel addition to the biobrick library, the phsABC gene that is known to encode Thiosulfate Reductase.

(1) phsABC gene and vector
phsABC in pSB74

This central component encodes Thiosulfate Reductase. The gene phsABC was obtained from Dr. Jay Keasling laboratory at University of California, Berkeley. According to their results, Thiosulfate Reductase encoded in the plasmid pSB74 showed the highest activity, so we obtained phsABC from the plasmid pSB74. E. coli DH5α strain were used for plasmid manipulation.

Figure from Keasling’s research: Sulfide production by phsABC in various plasmids. pSB74 (orange) showed the highest reactivity.
Read more about the background of the PHS gene