Team:Wisconsin-Madison/results

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==Timed Lysis: Inducible/Repressible Promoter==
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Revision as of 17:56, 26 October 2010

Encapsulation

Parts used in Encapsulation Experiments
Part Number Function Expression Type Zip File
<partinfo>BBa_k318500</partinfo> Produces Trascription Factor RcsB Inducible - IPTG 500
<partinfo>BBa_k318501</partinfo> Produces Trascription Factor RcsA Inducible - IPTG 501
<partinfo>BBa_k318502</partinfo> Produces Trascription Factor RcsA & RcsB Inducible - IPTG 502
<partinfo>BBa_k200021</partinfo> Empty Vector/Contol Inducible - IPTG NA


Colonic Acid Quantification

Background

Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Biological extracts often contain compounds, which under heating with H2SO4 yield brown products absorbing between 396 nm and 427 nm. Colonic acid can be estimated by measuring L-fucose content.

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Results

Conclusion


Cell Survivability in Acidic Conditions

Background

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Results

Cell Survivability Testing Graphs.jpg

Conclusion


Timed Lysis

Parts used in these Experiments
Part Number Function Induction Zip File
<partinfo>BBa_k318513</partinfo> Produces RFP stationary phase 513

Background

gadAp: A pH Sensitive Promoter Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part

pH Induction Results

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Stationary Phase Induction Results

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Conclusion