Team:Wisconsin-Madison/results

From 2010.igem.org

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==Encapsulation==
==Encapsulation==
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{| border="1" cellpadding="5" cellspacing="0" align="center"
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|+ align="center" style="color:#000000;" |''Parts used in Encapsulation Experiments''
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|-
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|'''Part Number'''
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|'''Function'''
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|'''Expression Type'''
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|'''Zip File'''
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|-
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|<partinfo>BBa_k318500</partinfo>
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|Produces Trascription Factor RcsB
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|Inducible - IPTG
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|500
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|-
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|<partinfo>BBa_k318501</partinfo>
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|Produces Trascription Factor RcsA
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|Inducible - IPTG
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|501
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|-
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|<partinfo>BBa_k318502</partinfo>
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|Produces Trascription Factor RcsA & RcsB
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|Inducible - IPTG
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|502
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|-
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|<partinfo>BBa_k200021</partinfo>
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|Empty Vector/Contol
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|Inducible - IPTG
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|NA
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|}
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<br>
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===Colonic Acid Quantification===
===Colonic Acid Quantification===
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====Background====
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Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Biological extracts often contain compounds, which under heating with H2SO4 yield brown products absorbing between 396 nm and 427 nm. Colonic acid can be estimated by measuring L-fucose content.
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Download procedure [[Media:UW_colonicacidassay.pdf|here]] or continue to experimental protocol page
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====Results====
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===Cell Survivability in Acidic Conditions===
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====Background====
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-
===Encapsulation: Cell Survivability in Acidic Conditions===
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Download procedure [[Media:UW_???.pdf|here]] or continue to experimental protocol page
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====Results====
==Timed Lysis: Inducible/Repressible Promoter==
==Timed Lysis: Inducible/Repressible Promoter==

Revision as of 17:48, 26 October 2010

Contents

Encapsulation

Parts used in Encapsulation Experiments
Part Number Function Expression Type Zip File
<partinfo>BBa_k318500</partinfo> Produces Trascription Factor RcsB Inducible - IPTG 500
<partinfo>BBa_k318501</partinfo> Produces Trascription Factor RcsA Inducible - IPTG 501
<partinfo>BBa_k318502</partinfo> Produces Trascription Factor RcsA & RcsB Inducible - IPTG 502
<partinfo>BBa_k200021</partinfo> Empty Vector/Contol Inducible - IPTG NA


Colonic Acid Quantification

Background

Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Biological extracts often contain compounds, which under heating with H2SO4 yield brown products absorbing between 396 nm and 427 nm. Colonic acid can be estimated by measuring L-fucose content.

Download procedure here or continue to experimental protocol page

Results

Cell Survivability in Acidic Conditions

Background

Download procedure here or continue to experimental protocol page

Results

Timed Lysis: Inducible/Repressible Promoter

gadAp: A pH Sensitive Promoter

Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part

pH Induction

Stationary Phase Induction

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