"

From 2010.igem.org

Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:

• Genebank file will all features (.gbk???)
• Fwd and Reverse sequencing files (.ab1)
• Gels from cloning and confirmation (.pdf)
• All associated testing files and Results (.xls, .pdf, etc.)

List of Parts

Enzyme Delivery Testing Constructs

Part Number	Function	Expression Type	Zip File
<partinfo>BBa_k318500</partinfo>	Produces Trascription Factor RcsB	Inducible - IPTG	500
<partinfo>BBa_k318501</partinfo>	Produces Trascription Factor RcsA	Inducible - IPTG	501
<partinfo>BBa_k318502</partinfo>	Produces Trascription Factor RcsA & RcsB	Inducible - IPTG	502

Enzyme Delivery Final Constructs

Part Number	Function	Expression Type
<partinfo>BBa_k318500</partinfo>	Produces Trascription Factors RcsA & RcsB	Constitutive
<partinfo>BBa_k318500</partinfo>	Produces Beta-Galactasidase	Constitutive
<partinfo>BBa_k318500</partinfo>	A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA)	pH 2
<partinfo>BBa_k318500</partinfo>	A) Activates Lysis once in the small intestine	(+)-LuxR, (-)-cILVA
<partinfo>BBa_k318500</partinfo>	B) Activates lysis once in the small intestine	Bile Salts

Team Favorites

The Key Plasmid

BBa_K318000

Other Parts

BBa_K318010
BBa_K318011
BBa_K318012
BBa_K318013
BBa_K318014

Characterization

1. Part 1
2. Part 2

Part Files

Put your .zip files here:

(Remember to include a Genbank file, part description, gel images (if available), sequencing information, and any characterization information)