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From 2010.igem.org

New Standards

Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:

• Genbank including features and plasmid (.gbk???)
• Fwd and Reverse sequencing files (.ab1)
• Gels from cloning and confirmation (.pdf)
• All associated testing files and Results (.xls, .pdf, etc.)

Enzyme Delivery

New DNA

Enzyme Delivery Testing Constructs

Part Number	About	Testing in...	Zip File
<partinfo>BBa_k318512</partinfo>	Regulatory - pH inducible promoter	<partinfo>BBa_k318513</partinfo>	512
<partinfo>BBa_k318514</partinfo>	Gene - RamA protein can bind to cholic acid (bile salts)	<partinfo>BBa_k318515</partinfo>	514
<partinfo>BBa_k318516</partinfo>	Regulatory - RamA/Bile-salt conjugate inducible promoter	<partinfo>BBa_k318517</partinfo>	516

Testing Constructs

Enzyme Delivery Testing Constructs

Part Number	Function	Expression Type	Zip File
<partinfo>BBa_k318500</partinfo>	Produces Trascription Factor RcsB	Inducible - IPTG	500
<partinfo>BBa_k318501</partinfo>	Produces Trascription Factor RcsA	Inducible - IPTG	501
<partinfo>BBa_k318502</partinfo>	Produces Trascription Factor RcsA & RcsB	Inducible - IPTG	502
<partinfo>BBa_k318503</partinfo>	Produces Trascription Factor YgiV - Blocks Biofilm	Inducible - IPTG	503
<partinfo>BBa_k318504</partinfo>	Produces RfaI gene - WaaI Ligase	Inducible - IPTG	504
<partinfo>BBa_k318505</partinfo>	Produces T.F. YgiV & RfaI gene	Inducible - IPTG	505
<partinfo>BBa_k318506</partinfo>	Produces RFP	(+)LuxR, (-)cILVA	506
<partinfo>BBa_k318509</partinfo>	Produces Repressor cI-LVA (degredation tag)	Inducible - IPTG	509
<partinfo>BBa_k318510</partinfo>	Produces Repressor cI (no degredation tag)	Inducible - IPTG	510
<partinfo>BBa_k318511</partinfo>	Produces Activator LuxR	Anhydrous Tetrycycline	511
<partinfo>BBa_k318513</partinfo>	Produces RFP	pH	513
<partinfo>BBa_k318515</partinfo>	Produces Trascription Factor RcsA & RcsB	RamA/Bile-Salt Complex	515
<partinfo>BBa_k318517</partinfo>	Produces RamA gene	Bile Salt	517

Final Constructs

Enzyme Delivery Final Constructs

Part Number	Function	Expression Type
<partinfo>BBa_k3185##</partinfo>	Produces Trascription Factors RcsA & RcsB, and B-Gal	Constitutive
<partinfo>BBa_k3185##</partinfo>	A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA)	pH 2
<partinfo>BBa_k318507</partinfo>	A) Activates Lysis once in the small intestine	(+)-LuxR, (-)-cILVA
<partinfo>BBa_k318519</partinfo>	B) Activates lysis once in the small intestine	Bile Salts

Encryption

Team Favorites

The Key Plasmid

BBa_K318000

Other Parts

BBa_K318010
BBa_K318011
BBa_K318012
BBa_K318013
BBa_K318014

Part Files

Put your .zip files here:

(Remember to include a Genbank file, part description, gel images (if available), sequencing information, and any characterization information)