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Team:Wisconsin-Madison/parts
From 2010.igem.org
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==New Standards== | ==New Standards== | ||
Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following: | Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following: | ||
| - | * | + | * Genbank including features and plasmid (.gbk???) |
* Fwd and Reverse sequencing files (.ab1) | * Fwd and Reverse sequencing files (.ab1) | ||
* Gels from cloning and confirmation (.pdf) | * Gels from cloning and confirmation (.pdf) | ||
* All associated testing files and Results (.xls, .pdf, etc.) | * All associated testing files and Results (.xls, .pdf, etc.) | ||
| - | + | <br> | |
==Enzyme Delivery== | ==Enzyme Delivery== | ||
===<center>Testing Constructs</center>=== | ===<center>Testing Constructs</center>=== | ||
| Line 47: | Line 47: | ||
|Inducible - IPTG | |Inducible - IPTG | ||
|502 | |502 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318503</partinfo> | ||
| + | |Produces Trascription Factor YgiV - Blocks Biofilm | ||
| + | |Inducible - IPTG | ||
| + | |503 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318504</partinfo> | ||
| + | |Produces RfaI gene - WaaI Ligase | ||
| + | |Inducible - IPTG | ||
| + | |504 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318505</partinfo> | ||
| + | |Produces T.F. YgiV & RfaI gene | ||
| + | |Inducible - IPTG | ||
| + | |505 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318506</partinfo> | ||
| + | |Produces RFP | ||
| + | |(+)LuxR, (-)cILVA | ||
| + | |506 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318509</partinfo> | ||
| + | |Produces Repressor cI-LVA (degredation tag) | ||
| + | |Inducible - IPTG | ||
| + | |509 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318510</partinfo> | ||
| + | |Produces Repressor cI (no degredation tag) | ||
| + | |Inducible - IPTG | ||
| + | |510 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318511</partinfo> | ||
| + | |Produces Activator LuxR | ||
| + | |Anhydrous Tetrycycline | ||
| + | |511 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318513</partinfo> | ||
| + | |Produces RFP | ||
| + | |pH | ||
| + | |513 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318515</partinfo> | ||
| + | |Produces Trascription Factor RcsA & RcsB | ||
| + | |RamA/Bile-Salt Complex | ||
| + | |515 | ||
| + | |- | ||
| + | |<partinfo>BBa_k318517</partinfo> | ||
| + | |Produces RamA gene | ||
| + | |Bile Salt | ||
| + | |517 | ||
|} | |} | ||
| Line 69: | Line 119: | ||
|pH 2 | |pH 2 | ||
|- | |- | ||
| - | |<partinfo> | + | |<partinfo>BBa_k318507</partinfo> |
|A) Activates Lysis once in the small intestine | |A) Activates Lysis once in the small intestine | ||
|(+)-LuxR, (-)-cILVA | |(+)-LuxR, (-)-cILVA | ||
Revision as of 01:24, 28 September 2010
New Standards
Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:
- Genbank including features and plasmid (.gbk???)
- Fwd and Reverse sequencing files (.ab1)
- Gels from cloning and confirmation (.pdf)
- All associated testing files and Results (.xls, .pdf, etc.)
Enzyme Delivery
Testing Constructs
| Part Number | Function | Expression Type | Zip File |
| <partinfo>BBa_k318500</partinfo> | Produces Trascription Factor RcsB | Inducible - IPTG | 500 |
| <partinfo>BBa_k318501</partinfo> | Produces Trascription Factor RcsA | Inducible - IPTG | 501 |
| <partinfo>BBa_k318502</partinfo> | Produces Trascription Factor RcsA & RcsB | Inducible - IPTG | 502 |
| <partinfo>BBa_k318503</partinfo> | Produces Trascription Factor YgiV - Blocks Biofilm | Inducible - IPTG | 503 |
| <partinfo>BBa_k318504</partinfo> | Produces RfaI gene - WaaI Ligase | Inducible - IPTG | 504 |
| <partinfo>BBa_k318505</partinfo> | Produces T.F. YgiV & RfaI gene | Inducible - IPTG | 505 |
| <partinfo>BBa_k318506</partinfo> | Produces RFP | (+)LuxR, (-)cILVA | 506 |
| <partinfo>BBa_k318509</partinfo> | Produces Repressor cI-LVA (degredation tag) | Inducible - IPTG | 509 |
| <partinfo>BBa_k318510</partinfo> | Produces Repressor cI (no degredation tag) | Inducible - IPTG | 510 |
| <partinfo>BBa_k318511</partinfo> | Produces Activator LuxR | Anhydrous Tetrycycline | 511 |
| <partinfo>BBa_k318513</partinfo> | Produces RFP | pH | 513 |
| <partinfo>BBa_k318515</partinfo> | Produces Trascription Factor RcsA & RcsB | RamA/Bile-Salt Complex | 515 |
| <partinfo>BBa_k318517</partinfo> | Produces RamA gene | Bile Salt | 517 |
Final Constructs
| Part Number | Function | Expression Type |
| <partinfo>BBa_k318500</partinfo> | Produces Trascription Factors RcsA & RcsB | Constitutive |
| <partinfo>BBa_k318500</partinfo> | Produces Beta-Galactasidase | Constitutive |
| <partinfo>BBa_k318500</partinfo> | A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) | pH 2 |
| <partinfo>BBa_k318507</partinfo> | A) Activates Lysis once in the small intestine | (+)-LuxR, (-)-cILVA |
| <partinfo>BBa_k318500</partinfo> | B) Activates lysis once in the small intestine | Bile Salts |
Encryption
Team Favorites
The Key Plasmid
Other Parts
BBa_K318010
BBa_K318011
BBa_K318012
BBa_K318013
BBa_K318014
Part Files
Put your .zip files here:
(Remember to include a Genbank file, part description, gel images (if available), sequencing information, and any characterization information)
