| Mutant
Name |
Brief Description |
Features and Mutations |
Transpeptidation per DCP |
Hydrolysis per DCP |
| CapD |
A protein native to Bacillus anthracis which
cleaves poly-D-γ-glutamate (PDGA) and anchors it to the bacterium's
peptidoglycan |
No Signal Sequence, Triple
band on protein agarose gel |
0.491 |
0.713 |
| CapD_CP |
A circularly permuted CapD
with a Foldit-designed linker |
Foldit created linker,
Circular Permutation, No Signal Sequence, Single band on protein agarose gel |
1.000 |
1.000 |
| CapD_CPNFDelta |
A circularly permuted CapD
with deletion of floppy areas |
Foldit created linker,
Circular Permutation, No Signal Sequence, Single band on protein agarose gel,
Removed "floppy" section to compact protein |
1.035 |
0.878 |
| F24A |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
24 on CapDCP from Phenylalanine to Alanine, Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.021 |
0.061 |
| F24H |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
24 on CapDCP from Phenylalanine to Histidine, Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.108 |
1.205 |
| F24H,
L40R, T59D_M61S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24, 40, 59, and 61 on Foldit design from Phenylalanine to Histidine, Leucine
to Arginine, Threonine to Asparagine, and Methionine to Serine, respectively.
Foldit-created linker, Circular Permutation, No Signal Sequence, Single band
on protein agarose gel |
0.001 |
0.006 |
| F24H,
L40R, T59N_M61S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24, 40, 59, and 61 on Foldit design from Phenylalanine to Histidine, Leucine
to Arginine, Threonine to Aspartic Acid, and Methionine to Serine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.058 |
1.050 |
| F24H,
L40R, T59S_M61S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24, 40, 59, and 61 on CapDCP from Phenylalanine to Histidine, Leucine to
Arginine, Threonine to Serine, and Methionine to Serine, respectively.
Foldit-created linker, Circular Permutation, No Signal Sequence, Single band
on protein agarose gel |
0.000 |
0.007 |
| F24H,
R356K |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24 and 356 on CapDCP from Phenylalanine to Histidine and Arginine to Lysine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.129 |
0.257 |
| F24H,
T59R |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24 and 59 on CapDCPfrom Phenylalanine to Histidine and Threonine to Arginine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.011 |
0.005 |
| F24H,T59N |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24 and 59 on CapDCP from Phenylalanine to Histidine and Threonine to
Asparagine, respectively. Foldit-created linker, Circular Permutation, No
Signal Sequence, Single band on protein agarose gel |
0.007 |
0.029 |
| F24W |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
24 on CapDCP from Phenylalanine to Tryptophan. Foldit-created linker,
Circular Permutation, No Signal Sequence, Single band on protein agarose gel |
0.006 |
0.044 |
| F24Y |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
24 on CapDCP from Phenylalanine to Tyrosine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.593 |
0.887 |
| F24Y,
L40R |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24 and 40 on CapDCP from Phenylalanine to Tyrosine and Leucine to Arginine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.009 |
-0.013 |
| F24Y,
L40R, T59N |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24, 40, 59 on CapDCP from Phenylalanine to Tyrosine, Leucine to
Arginine, and Threonine to Asparagine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.003 |
0.157 |
| F24Y,R305K |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24 and 305 on CapDCP from Phenylalanine to Tyrosine and Arginine to Lysine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.002 |
0.233 |
| F24Y,T59N |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24 and 59 on CapDCP from Phenylalanine to Tyrosine and Threonine to
Asparagine, respectively. Foldit-created linker, Circular Permutation, No
Signal Sequence, Single band on protein agarose gel |
0.008 |
0.165 |
| F24Y,T59N,R305K |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
24, 59, and 356 on CapDCP from Phenylalanine to Tyrosine, Threonine to
Asparagine, and Arginine to Lysine, respectively. Foldit-created linker,
Circular Permutation, No Signal Sequence, Single band on protein agarose gel |
0.002 |
0.127 |
| F60W |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
60 on CapDCP from Phenylalanine to Tryptophan. Foldit-created linker,
Circular Permutation, No Signal Sequence, Single band on protein agarose gel |
0.239 |
0.489 |
| G79A |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
79 on CapDCP from Glycine to Alanine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.040 |
-0.511 |
| I83T |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
83 on CapDCP from Isoleucine to Threonine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.009 |
0.006 |
| I83V |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
83 on CapDCP from Isoleucine to Valine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.003 |
0.043 |
| L40A |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
40 on CapDCP from Leucine to Alanine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.150 |
0.413 |
| L40R |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
40 on CapDCP from Leucine to Arginine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
1.157 |
1.527 |
| L40S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
40 on CapDCP from Leucine to Serine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.166 |
0.577 |
| L40W |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
40 on CapDCP from Leucine to Tryptophan. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.825 |
1.215 |
| M61H |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
61 on CapDCP from Methionine to Histidine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
-0.003 |
0.002 |
| M61N |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
61 on CapDCP from Methionine to Asparagine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.011 |
0.002 |
| M61S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
61 on CapDCP from Methionine to Serine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.262 |
0.485 |
| N23K |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
23 on CapDCP from Asparagine to Lysine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.818 |
0.839 |
| N23Q |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
23 on CapDCP from Asparagine to Glutamine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.165 |
0.646 |
| S143K |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
143 on CapDCP from Serine to Lysine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.574 |
0.426 |
| S22I,
T59A |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
22 and 59 on CapDCP from Serine to Isoleucine and Threonine to Alanine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.030 |
-0.057 |
| S22Q,
T59Q |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
22 and 59 on CapDCP from Serine to Glutamine and Threonine to Glutamine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
-0.012 |
0.085 |
| S57H,
M61H |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
57 and 61 on CapDCP from Serine to Histidine and Methionine to Histidine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
-0.004 |
-0.007 |
| T18L,
T59Q, M61N, F452W |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
18, 59, 61, and 452 on CapDCP from Threonine to Leucine, Threonine to
Glutamine, Methionine to Asparagine, and Phenylalanine to Tryptophan, respectively. Foldit-created linker,
Circular Permutation, No Signal Sequence, Single band on protein agarose gel |
0.020 |
-0.028 |
| T18S_T20S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
18 and 20 on CapDCP from Threonine to Serine and Threonine to Serine, respectively. Foldit-created linker,
Circular Permutation, No Signal Sequence, Single band on protein agarose gel |
0.617 |
1.186 |
| T20A |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
20 on CapDCP from Threonine to Alanine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.011 |
0.021 |
| T20C |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
20 on CapDCP from Threonine to Cystine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.090 |
0.142 |
| T20S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
20 on CapDCP from Threonine to Serine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.639 |
1.443 |
| T20S,
L40D |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 40 on CapDCP from Threonine to Serine and Leucine to Asparagine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.853 |
0.001 |
| T20S,
L40E |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 40 on CapDCP from Threonine to Serine and Leucine to Glutamic Acid,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.163 |
0.637 |
| T20S,
L40F |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 40 on CapDCP from Threonine to Serine and Leucine to Phenylalanine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.246 |
0.335 |
| T20S,
L40H |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 40 on CapDCP from Threonine to Serine and Leucine to Histidine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.001 |
0.012 |
| T20S,
L40K |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 40 on CapDCP from Threonine to Serine and Leucine to Lysine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.000 |
0.007 |
| T20S,
L40Q |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 40 on CapDCP from Threonine to Serine and Leucine to Glutamine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.126 |
0.478 |
| T20S,
L40R |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 40 on CapDCP from Threonine to Serine and Leucine to Arginine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.000 |
0.009 |
| T20S,
L40R, F60E |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and
Phenylalanine to Glutamic Acid, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.048 |
0.028 |
| T20S,
L40R, F60Q |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and
Phenylalanine to Glutamine, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.002 |
0.007 |
| T20S,
L40R, N81E |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and
Phenylalanine to Glutamine, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.001 |
-0.004 |
| T20S,
L40R, N81Q |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 40, and 81 on CapDCP from Threonine to Serine, Leucine to Arginine, and
Asparagine to Glutamine, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.422 |
0.321 |
| T20S,
L40R, T59K |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 40, and 59 on CapDCP from Threonine to Serine, Leucine to Arginine, and
Threonine to Lysine, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.048 |
0.033 |
| T20S,
L40R, T59K_M61S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 40, 59, and 61 on CapDCP from Threonine to Serine, Leucine to Arginine,
Threonine to Lysine, and Methionine to Serine, respectively. Foldit-created
linker, Circular Permutation, No Signal Sequence, Single band on protein agarose
gel |
0.010 |
0.032 |
| T20S,
T59Q, M61T |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to Glutamine,
and Methionine to Threonine, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.007 |
-0.069 |
| T20S,M61A |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Alanine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.005 |
0.045 |
| T20S,M61C |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Cystine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.102 |
0.207 |
| T20S,M61D |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Aspartic Acid,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.004 |
0.003 |
| T20S,M61G |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Glycine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.004 |
0.029 |
| T20S,M61L |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Leucine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.004 |
0.029 |
| T20S,M61N |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Asparagine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.003 |
0.002 |
| T20S,M61S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Serine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.007 |
0.053 |
| T20S,M61T |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Threonine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.038 |
0.363 |
| T20S,M61V |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 61 on CapDCP from Threonine to Serine and Methionine to Valine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.004 |
0.039 |
| T20S,T59N |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 59 on CapDCP from Threonine to Serine and Threonine to Asparagine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.019 |
0.125 |
| T20S,T59S_M61S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to Serine, and
Methionine to Serine, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.300 |
0.719 |
| T20S_F24H |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 24 on CapDCP from Threonine to Serine and Phenylalanine to Histidine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.008 |
0.097 |
| T20S_F24Y |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20 and 24 on CapDCP from Threonine to Serine and Phenylalanine to Tyrosine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.040 |
0.201 |
| T20S_F24Y,T59N |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
20, 24, and 59 on CapDCP from Threonine to Serine, Phenylalanine to Tyrosine,
and Threonine to Asparagine, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.013 |
0.048 |
| T2A |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue 2
on CapDCP from Threonine to Alanine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.007 |
-0.020 |
| T2C |
A mutated CapDCP protein
aimed at destroying all activity with poly-D-gamma-glutamate. |
Point mutation at residue 2
on CapDCP from Threonine to Cystine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.001 |
-0.005 |
| T2S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue 2
on CapDCP from Threonine to Serine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.024 |
0.325 |
| T2S,T18S_T20S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
2, 18, and 20 on CapDCP from Threonine to Serine, Threonine to Serine, and
Threonine to Serine, respectively. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.144 |
0.250 |
| T2S,T18S_T20S,T59S_M61S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
2, 18, 20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to
Serine, Threonine to Serine, Threonine to Serine and Threonine to Serine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.003 |
0.022 |
| T2S,T20S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
2 and 20 on CapDCP from Threonine to Serine and Threonine to Serine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.106 |
0.089 |
| T2V |
A mutated CapDCP protein
aimed at destroying all activity with poly-D-gamma-glutamate. |
Point mutation at residue 2
on CapDCP from Threonine to Valine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.000 |
0.000 |
| T59H_M61A |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
59 and 61 on CapDCP from Threonine to Histidine and Methionine to Alanine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.000 |
0.000 |
| T59H_M61T |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
59 and 61 on CapDCP from Threonine to Histidine and Methionine to Threonine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.000 |
0.000 |
| T59M |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
59 on CapDCP from Threonine to Methionine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.008 |
0.008 |
| T59N |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
59 on CapDCP from Threonine to Asparagine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.232 |
0.868 |
| T59Q |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
59 on CapDCP from Threonine to Glutamine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
0.000 |
0.000 |
| T59Q,
M61Q, F452W |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
59, 61, and 452 on CapDCP from Threonine to Glutamine, Threonine to
Glutamine, and Phenylalanine to Tryptophan, respectively. Foldit-created
linker, Circular Permutation, No Signal Sequence, Single band on protein
agarose gel |
-0.014 |
0.037 |
| T59S |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residue
59 on CapDCP from Threonine to Serine. Foldit-created linker, Circular
Permutation, No Signal Sequence, Single band on protein agarose gel |
3.525 |
1.047 |
| T59S,
M61H |
A mutated CapDCP protein
aimed at increasing hydrolysis and decreasing transpeptidation of
poly-D-gamma-glutamate. |
Point mutation at residues
59 and 61 on CapDCP from Threonine to Serine and Methionine to Histidine,
respectively. Foldit-created linker, Circular Permutation, No Signal
Sequence, Single band on protein agarose gel |
0.013 |
0.051 |
|
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