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Team:WashU/Notebook/Yeast
From 2010.igem.org
Week of 6/21
6/24
plated Yeast Gal-YFP MATa, trp1, leu2::GAL1-YFP URA3/Kanr and Yeast YF4 MATa Prototroph on a Kan plate and a YPD plate
Started a liquid culture of Yeast YF4 MATa Prototroph in YPD
Week of 6/28
6/29
A practice transformation was preformed at the Cohen Lab with Ilaria. The OD600 was 0.651 which was low, but used in order to speed up the experiment. The PMKV005 loxKanlox Plasmid (McCusker 2005 Yeast, 21: 163-171) was used into transform wildtype yeast. The plates used were Kan positive plates created on 6/24. However it was later found that these plates might not correctly select for drug resistance. Two plates were made, one with the transformant and one with a control. The attached procedure was used and annotated during the experiment
7/1
• Examined the Sample and Control plates from the practice transformation on June 29th. Two colonies had grown on the Sample plate and none showed on the control plate. The low colony number could be due to a low OD600 (0.651) used in the transformation. • Plated the two colonies on a G418 plate in order to conduct the first round of purification
