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Team:Warsaw/Stage1/Modeling
From 2010.igem.org
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Durig the brainstorm period of our project we came up with many interesting ideas and we tried to find a way to squeeze all those ideas into one E.Coli Cell. It lead us to one generel dilema. How many different genes can we insert into our Coli at one time andd how precisly regulate their dosage. We decides to use different RBS sequences to controol gene exporession. | Durig the brainstorm period of our project we came up with many interesting ideas and we tried to find a way to squeeze all those ideas into one E.Coli Cell. It lead us to one generel dilema. How many different genes can we insert into our Coli at one time andd how precisly regulate their dosage. We decides to use different RBS sequences to controol gene exporession. | ||
| + | |||
| + | We knew from the registry that B0033 is weak and for strong expresssion we should use B0034, but also we have learned that those RBSes were measures in one seup with standard promoter and GFP reporter gene. We faced two ganeral questios:</p> | ||
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| + | <p>1) Are those 5 measured RBSes in the registry enaugh? Does it make sense to to use any other RBSes?</p> | ||
| + | |||
| + | <p>2) RBSes were measured with GFP reporter. Are these RBS measurements universal. Would RBS behave differently if we pout our favourite gene uinstead of GFP? </p> | ||
| + | |||
| + | <p>We went downstairs to the cafeteria, ordered lots of coffe, switched on the laptops and asked google scholar for help. | ||
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</p> | </p> | ||
<img src="https://static.igem.org/mediawiki/2010/2/29/RBSpredict_ania5MDP.png"> | <img src="https://static.igem.org/mediawiki/2010/2/29/RBSpredict_ania5MDP.png"> | ||
Revision as of 15:50, 24 October 2010
Durig the brainstorm period of our project we came up with many interesting ideas and we tried to find a way to squeeze all those ideas into one E.Coli Cell. It lead us to one generel dilema. How many different genes can we insert into our Coli at one time andd how precisly regulate their dosage. We decides to use different RBS sequences to controol gene exporession. We knew from the registry that B0033 is weak and for strong expresssion we should use B0034, but also we have learned that those RBSes were measures in one seup with standard promoter and GFP reporter gene. We faced two ganeral questios:
1) Are those 5 measured RBSes in the registry enaugh? Does it make sense to to use any other RBSes?
2) RBSes were measured with GFP reporter. Are these RBS measurements universal. Would RBS behave differently if we pout our favourite gene uinstead of GFP?
We went downstairs to the cafeteria, ordered lots of coffe, switched on the laptops and asked google scholar for help.
Fig 1.
Fig 2.
