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Team:Warsaw/Calendar-Stage2/17 September 2010
From 2010.igem.org
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<p align="justify">Four colonies were picked for inoculation of liquid cultures. Cultures were grown for 6 h in 37 <sup>o</sup>C. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.</p> | <p align="justify">Four colonies were picked for inoculation of liquid cultures. Cultures were grown for 6 h in 37 <sup>o</sup>C. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.</p> | ||
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| - | <img | + | <img src="https://static.igem.org/mediawiki/2010/8/8f/Minc2_copy.jpg" height=400 width=400> |
| - | <p align=" | + | <p align="left"><i>C - unmutated plasmid; M - marker; 1,2,3,4 -respective samples</i></p> |
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<p align="justify">The shorter band in samples 1,2,3 appears to be slightly longer compared to control, indicating a succesfull mutagenesis. These conclusions were later verified by sequencing.</p> | <p align="justify">The shorter band in samples 1,2,3 appears to be slightly longer compared to control, indicating a succesfull mutagenesis. These conclusions were later verified by sequencing.</p> | ||
Revision as of 15:49, 25 October 2010
Mutagenesis of MinC
Kuba
Four colonies were picked for inoculation of liquid cultures. Cultures were grown for 6 h in 37 oC. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.
C - unmutated plasmid; M - marker; 1,2,3,4 -respective samples
The shorter band in samples 1,2,3 appears to be slightly longer compared to control, indicating a succesfull mutagenesis. These conclusions were later verified by sequencing.
