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Team:Warsaw/Calendar-Stage1/8 September 2010
From 2010.igem.org
(Difference between revisions)
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<br><div class="note"> Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]</div> | <br><div class="note"> Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]</div> | ||
<br>1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion | <br>1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion | ||
| - | <br>2. Preparation of | + | <br>2. Preparation of samples and examining them in flow cytometer |
<br> | <br> | ||
<br><div class="note"> Data analysis with Cell Quest[Cherry]</div> | <br><div class="note"> Data analysis with Cell Quest[Cherry]</div> | ||
| Line 31: | Line 31: | ||
<br />07-09-2010.007- pH 4,8 | <br />07-09-2010.007- pH 4,8 | ||
<br />07-09-2010.010- pH 8 | <br />07-09-2010.010- pH 8 | ||
| - | <br /> | + | |
| - | <br /><hr /> | + | <br /><br />cytometer plots: |
| + | <br>FSC- Forward Scatter, cell size | ||
| + | <br>SSC- Side Scatter, cell complexity | ||
| + | <br>FL-1- FL-1 sensor, cell fluorescence | ||
| + | <br /><br><hr /> | ||
<br /> | <br /> | ||
Data from Cell Quest analysis: | Data from Cell Quest analysis: | ||
Revision as of 20:27, 27 October 2010
07.09.2010 Tuesday
Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]
1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion
2. Preparation of samples and examining them in flow cytometer
Data analysis with Cell Quest[Cherry]
legend:
07-09-2010.004- pH 7
07-09-2010.005- pH 8
07-09-2010.006- pH 6,8
07-09-2010.007- pH 4,8
07-09-2010.010- pH 8
cytometer plots:
FSC- Forward Scatter, cell size
SSC- Side Scatter, cell complexity
FL-1- FL-1 sensor, cell fluorescence
Data from Cell Quest analysis:
