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Team:Warsaw/Calendar-Stage1/7 July 2010
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(New page: <html> <script type="text/javascript"> ActiveTab='WetLab'; </script> </html> {{TemplateTop}} {{UpperTabs}} {{LowerTabsTeam}} <html> <!-- do not edit above me! --> <br> 07.07.2010 Wednesd...) |
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<br> 1. Cultures from 6.07.2010 were centrifuged -> sediments in the eppendorfs were light green. | <br> 1. Cultures from 6.07.2010 were centrifuged -> sediments in the eppendorfs were light green. | ||
| - | <br> 2. Transformation results from 6.07.2010: | + | <br> 2. Alkaline lysis of these 'green cultures'. |
| - | + | <br> 3. Inoculation of Petri plates with ampicillin. | |
| - | <br> | + | <br> |
| - | + | <br> -> Transformation results from 6.07.2010: clones were green during exposition to UV light -> we inoculated the plates with ampicillin. | |
| + | <br> | ||
| + | <br><div class="note">Cloning GFP-terminator behind RBSes - approach 2. [Ania S., Ania P., Kasia, Cherry] </div> | ||
| + | <br> 1. Clones obtained on the plates after transformation were used to set up liquid cultures (per 2 ml). We've got 10-20 tubes with cultures from each plate (B0030/GFP = our number 7, B0031/GFP = our number 8, B0032/GFP = our number 9, B0033/GFP = our number 10, B0034/GFP = our number 11) | ||
Latest revision as of 14:56, 31 July 2010
07.07.2010 Wednesday
Cloning GFP-terminator behind RBSes - approach 1 [Ania S., Ania P., Kasia, Cherry]
1. Cultures from 6.07.2010 were centrifuged -> sediments in the eppendorfs were light green.
2. Alkaline lysis of these 'green cultures'.
3. Inoculation of Petri plates with ampicillin.
-> Transformation results from 6.07.2010: clones were green during exposition to UV light -> we inoculated the plates with ampicillin.
Cloning GFP-terminator behind RBSes - approach 2. [Ania S., Ania P., Kasia, Cherry]
1. Clones obtained on the plates after transformation were used to set up liquid cultures (per 2 ml). We've got 10-20 tubes with cultures from each plate (B0030/GFP = our number 7, B0031/GFP = our number 8, B0032/GFP = our number 9, B0033/GFP = our number 10, B0034/GFP = our number 11)
