Team:Warsaw/Calendar-Stage1/29 August 2010
From 2010.igem.org
legend:
E.coli constructs:
G- GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP
TOP- without construct
LIZ- treated with lysozyme
TRIT- treated with triton
X- untreated
bacteria:
30-08-2010.001- TOP
30-08-2010.003- G
30-08-2010.004- J
30-08-2010.005- A
Hela cells with bacteria:
30-08-2010.033- X TRIT
30-08-2010.034- A TRIT
30-08-2010.035- J TRIT
30-08-2010.036- A LIZ
30-08-2010.037- J LIZ
30-08-2010.038- without bacteria TRIT
30-08-2010.039- TOP TRIT
30-08-2010.040- G TRIT
30-08-2010.041- X LIZ
30-08-2010.042- without bacteria LIZ
30-08-2010.043- TOP LIZ
30-08-2010.044- G LIZ
Data from Cell Quest processed with Microsoft Excel:
Lysozyme | ||||
TOP | G | JPH | APH | |
M1 | 93,52% | 72,07% | 13,85% | 11,57% |
M2 | 6,22% | 4,93% | 33,06% | 21,72% |
M3 | 0,04% | 5,72% | 38,96% | 48,82% |
M4 | 7,33% | 15,32% | 86,74% | 87,04% |
Triton | ||||
TOP | G | JPH | APH | |
M1 | 88,89% | 85,60% | 9,13% | 13,47% |
M2 | 10,46% | 4,04% | 50,77% | 30,84% |
M3 | 0,07% | 2,73% | 21,95% | 27,61% |
M4 | 12,36% | 9,39% | 91,38% | 86,49% |