http://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&feed=atom&action=historyTeam:Warsaw/Calendar-Stage1/29 August 2010 - Revision history2024-03-28T21:16:25ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=195042&oldid=prevMlower: Team:Warsaw/Calendar-Stage2/30 August 2010 moved to Team:Warsaw/Calendar-Stage1/29 August 20102010-10-27T20:23:45Z<p><a href="/Team:Warsaw/Calendar-Stage2/30_August_2010" class="mw-redirect" title="Team:Warsaw/Calendar-Stage2/30 August 2010">Team:Warsaw/Calendar-Stage2/30 August 2010</a> moved to <a href="/Team:Warsaw/Calendar-Stage1/29_August_2010" title="Team:Warsaw/Calendar-Stage1/29 August 2010">Team:Warsaw/Calendar-Stage1/29 August 2010</a></p>
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<td colspan='1' style="background-color: white; color:black;">← Older revision</td>
<td colspan='1' style="background-color: white; color:black;">Revision as of 20:23, 27 October 2010</td>
</tr></table>Mlowerhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=194912&oldid=prevMlower: Team:Warsaw/Calendar-Stage3/30 August 2010 moved to Team:Warsaw/Calendar-Stage2/30 August 20102010-10-27T20:16:48Z<p><a href="/Team:Warsaw/Calendar-Stage3/30_August_2010" class="mw-redirect" title="Team:Warsaw/Calendar-Stage3/30 August 2010">Team:Warsaw/Calendar-Stage3/30 August 2010</a> moved to <a href="/Team:Warsaw/Calendar-Stage2/30_August_2010" class="mw-redirect" title="Team:Warsaw/Calendar-Stage2/30 August 2010">Team:Warsaw/Calendar-Stage2/30 August 2010</a></p>
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<td colspan='1' style="background-color: white; color:black;">← Older revision</td>
<td colspan='1' style="background-color: white; color:black;">Revision as of 20:16, 27 October 2010</td>
</tr></table>Mlowerhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=194852&oldid=prevCzeriberi at 20:13, 27 October 20102010-10-27T20:13:44Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><div class="note"> Previous day: Set up liquid cultures <del class="diffchange diffchange-inline">of different types </del>of E.coli [Ania P.]</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><div class="note"> Previous day: Set up liquid cultures of E.coli <ins class="diffchange diffchange-inline">with different constructs </ins>[Ania P.]</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>1. Incubation of Hela cells with different E.coli <del class="diffchange diffchange-inline">types</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>1. Incubation of Hela cells with different E.coli <ins class="diffchange diffchange-inline">with different constructs</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2. Preparation of samples with incubated cells and examining them in flow cytometer.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2. Preparation of samples with incubated cells and examining them in flow cytometer.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard, but with an extra version where we used Triton instead of lysozyme(<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> points 6 and 7)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard, but with an extra version where we used Triton instead of lysozyme(<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> points 6 and 7)<br></div></td></tr>
</table>Czeriberihttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=194675&oldid=prevCzeriberi at 20:07, 27 October 20102010-10-27T20:07:56Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>1. Incubation of Hela cells with different E.coli types. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>1. Incubation of Hela cells with different E.coli types. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2. Preparation of samples with incubated cells and examining them in flow cytometer.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>2. Preparation of samples with incubated cells and examining them in flow cytometer.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> points 6 and 7)<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard<ins class="diffchange diffchange-inline">, but with an extra version where we used Triton instead of lysozyme</ins>(<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> points 6 and 7)<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>legend:</strong></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>legend:</strong></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><hr /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><hr /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br /><strong>E.coli constructs:</strong></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br /><strong>E.coli <ins class="diffchange diffchange-inline">TOP 10 </ins>constructs:</strong></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />G- GFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />G- GFP</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />J- J23102+Listeriolysin O+Invasin+GFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />J- J23102+Listeriolysin O+Invasin+GFP</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />A- pAraC+Listeriolysin O+Invasin+GFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />A- pAraC+Listeriolysin O+Invasin+GFP</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br />TOP <del class="diffchange diffchange-inline">10</del>- without construct</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br />TOP- <ins class="diffchange diffchange-inline">TOP10 </ins>without construct</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />LIZ- treated with lysozyme</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />LIZ- treated with lysozyme</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>bacteria:</strong></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>bacteria:</strong></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br />30-08-2010.001- <del class="diffchange diffchange-inline">TOP 10</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br />30-08-2010.001- <ins class="diffchange diffchange-inline">TOP10 without construct</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.003- G</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.003- G</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.004- J</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.004- J</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>SSC- Side Scatter, cell complexity</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>SSC- Side Scatter, cell complexity</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>FL-1- FL-1 sensor, cell fluorescence</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>FL-1- FL-1 sensor, cell fluorescence</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><hr /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><hr /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>Cytometer data from Cell Quest analysis:</strong></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>Cytometer data from Cell Quest analysis:</strong></div></td></tr>
</table>Czeriberihttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=194582&oldid=prevCzeriberi at 20:04, 27 October 20102010-10-27T20:04:53Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>1. Incubation of Hela cells with different E.coli types. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>1. Incubation of Hela cells with different E.coli types. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>2. Preparation of <del class="diffchange diffchange-inline">probes </del>with incubated cells and examining them in flow cytometer.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>2. Preparation of <ins class="diffchange diffchange-inline">samples </ins>with incubated cells and examining them in flow cytometer.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> points 6 and 7)<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> points 6 and 7)<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.043- TOP LIZ</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.043- TOP LIZ</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.044- G LIZ</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.044- G LIZ</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br /><ins class="diffchange diffchange-inline"><br />cytometer plots:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br>FSC- Forward Scatter, cell size</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br>SSC- Side Scatter, cell complexity</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br>FL-1- FL-1 sensor, cell fluorescence</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><hr /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><hr /></div></td></tr>
</table>Czeriberihttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=191828&oldid=prevCzeriberi at 18:37, 27 October 20102010-10-27T18:37:24Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>E.coli constructs:</strong></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>E.coli constructs:</strong></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />G- GFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />G- GFP</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br />J- <del class="diffchange diffchange-inline">JPH</del>+Listeriolysin O+Invasin+GFP</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br />J- <ins class="diffchange diffchange-inline">J23102</ins>+Listeriolysin O+Invasin+GFP</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br />A- <del class="diffchange diffchange-inline">APH</del>+Listeriolysin O+Invasin+GFP</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br />A- <ins class="diffchange diffchange-inline">pAraC</ins>+Listeriolysin O+Invasin+GFP</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />TOP 10- without construct</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />TOP 10- without construct</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /></div></td></tr>
</table>Czeriberihttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=191701&oldid=prevCzeriberi at 18:34, 27 October 20102010-10-27T18:34:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>TOP- Hela cels with regular TOP E.coli</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>TOP- Hela cels with regular TOP E.coli</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>M1,M2,M3,M4- <del class="diffchange diffchange-inline">%of events gated with markers M from total</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>M1,M2,M3,M4- <ins class="diffchange diffchange-inline">PIN Units (according to <a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 7)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><img src="https://static.igem.org/mediawiki/2010/4/4e/Bacto.30.08_08_table.png" /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><img src="https://static.igem.org/mediawiki/2010/4/4e/Bacto.30.08_08_table.png" /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Czeriberihttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=191577&oldid=prevCzeriberi at 18:30, 27 October 20102010-10-27T18:30:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />J- JPH+Listeriolysin O+Invasin+GFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />J- JPH+Listeriolysin O+Invasin+GFP</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />A- APH+Listeriolysin O+Invasin+GFP</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />A- APH+Listeriolysin O+Invasin+GFP</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br />TOP- without construct</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br />TOP <ins class="diffchange diffchange-inline">10</ins>- without construct</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />LIZ- treated with lysozyme</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />LIZ- treated with lysozyme</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>bacteria:</strong></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br /><strong>bacteria:</strong></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br />30-08-2010.001- TOP</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br />30-08-2010.001- TOP <ins class="diffchange diffchange-inline">10</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.003- G</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.003- G</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.004- J</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br />30-08-2010.004- J</div></td></tr>
</table>Czeriberihttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=191469&oldid=prevCzeriberi at 18:27, 27 October 20102010-10-27T18:27:30Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br>Note:We improved the way of cell preparation by using lysozyme or triton to reduce the adherence of cells</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br>Note:We improved the way of cell preparation by using lysozyme or triton to reduce the adherence of cells</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br> <del class="diffchange diffchange-inline">Use </del>a <del class="diffchange diffchange-inline">protocol..</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br> <ins class="diffchange diffchange-inline">Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<</ins>a <ins class="diffchange diffchange-inline">href="http://openwetware</ins>.<ins class="diffchange diffchange-inline">org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><div class="note"> Previous day: Set up liquid cultures of different types of E.coli [<del class="diffchange diffchange-inline">Marta B</del>.]</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><div class="note"> Previous day: Set up liquid cultures of different types of E.coli [<ins class="diffchange diffchange-inline">Ania P</ins>.]</div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br> <del class="diffchange diffchange-inline">Use a </del>protocol.<del class="diffchange diffchange-inline">..</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br> <ins class="diffchange diffchange-inline">Conducted according to the </ins>protocol <ins class="diffchange diffchange-inline">included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware</ins>.<ins class="diffchange diffchange-inline">org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>1. Incubation of Hela cells with different E.coli types</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>1. Incubation of Hela cells with different E.coli types<ins class="diffchange diffchange-inline">. </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>2. Preparation of probes with incubated cells <del class="diffchange diffchange-inline">(using lysozyme and Triton) </del>and examining them in flow cytometer</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>2. Preparation of probes with incubated cells and examining them in flow cytometer<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br>Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> points 6 and 7)</ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>Czeriberihttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Stage1/29_August_2010&diff=167710&oldid=prevCzeriberi at 22:17, 26 October 20102010-10-26T22:17:14Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> 30.08.2010 Monday</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> 30.08.2010 Monday</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>Note:We improved the way of cell preparation by using lysozyme or triton to reduce the adherence of cells</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins><br>Note:We improved the way of cell preparation by using lysozyme or triton to reduce the adherence of cells</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Use a protocol...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br> Use a protocol...</div></td></tr>
</table>Czeriberi