Team:Warsaw/Calendar-Stage1/24 July 2010

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23.07.2010 Friday

Previous 2 days: Set up and culturing Hela cell culture [Kamil]

Use a protocol...

Previous day: Set up liquid cultures of different types of E.coli [Ania P.]

Use a protocol...

Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]

1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells and examining them in flow cytometer

Data analysis with Cell Quest and Microsoft Excel [Cherry]


legend:

E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP

Hela cells with bacteria:
23-07-2010.007- without bacteria
23-07-2010.018- with G (1)
23-07-2010.019- with G (2)
23-07-2010.020- with G (3)
23-07-2010.015- with I (1)
23-07-2010.016- with I (2)
23-07-2010.017- with I (3)
23-07-2010.012- with J (1)
23-07-2010.013- with J (2)
23-07-2010.014- with J (3)
23-07-2010.008- with A (1)
23-07-2010.010- with A (2)
23-07-2010.011- with A (3)




Data processed with Cell Quest:







Data from Cell Quest processed with Microsoft Excel:



Hela N
M186,66
M264,93
M33,3
M496,59
g1g2g3
32,1934,2730,73
47,45343
28,0926,2429,2
50,6355,447,16
MEANSDERR.
32,41,785,49
47,85,0110,49
27,841,55,37
51,064,148,1
i1i2i3
35,0637,8539,18
48,6655,1956,39
26,4525,2623,72
53,4159,4860,46
MEANSDERR.
37,362,15,63
53,414,167,79
25,141,375,44
57,783,826,61


 
M1
M2
M3
M4
j1j2j3
8,0116,038,49
28,5139,3529,22
59,2452,361,5
24,1537,6624,79
MEANSDERR.
10,844,541,48
32,366,0618,74
57,684,798,31
28,877,6226,4
a1a2a3
18,7810,078,84
46,4529,6830,58
43,1553,0556,19
44,2926,4826,31
MEANSDERR.
12,565,4243,13
35,579,4326,52
50,86,8113,4
32,3610,3331,93




  Hela N Hela G     Hela I     Hela J     Hela A    
    MEAN SD bląd MEAN SD ERR. MEAN SD ERR. MEAN SD ERR.
M1 86,66 32,40 1,78 5,49 37,36 2,10 5,63 10,84 4,50 41,48 12,56 5,42 43,13
M2 64,93 47,80 5,01 10,49 53,41 4,16 7,79 32,36 6,06 18,74 35,57 9,43 26,52
M3 3,30 27,84 1,50 5,37 25,14 1,37 5,44 57,68 4,79 8,31 50,80 6,81 13,40
M4 96,59 51,06 4,14 8,10 57,78 3,82 6,61 28,87 7,62 26,40 32,36 10,33 31,93