Team:Warsaw/Calendar-Stage1/24 July 2010
From 2010.igem.org
23.07.2010 Friday
Previous 2 days: Set up and culturing Hela cell culture [Kamil]
Use a protocol...
Previous day: Set up liquid cultures of different types of E.coli [Ania P.]
Use a protocol...
Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]
1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells and examining them in flow cytometer
Data analysis with Cell Quest and Microsoft Excel [Cherry]
legend:
E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP
Hela cells with bacteria:
23-07-2010.007- without bacteria
23-07-2010.018- with G (1)
23-07-2010.019- with G (2)
23-07-2010.020- with G (3)
23-07-2010.015- with I (1)
23-07-2010.016- with I (2)
23-07-2010.017- with I (3)
23-07-2010.012- with J (1)
23-07-2010.013- with J (2)
23-07-2010.014- with J (3)
23-07-2010.008- with A (1)
23-07-2010.010- with A (2)
23-07-2010.011- with A (3)
Data processed with Cell Quest:
Data from Cell Quest processed with Microsoft Excel:
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Hela N | Hela G | Hela I | Hela J | Hela A | |||||||||
MEAN | SD | bląd | MEAN | SD | ERR. | MEAN | SD | ERR. | MEAN | SD | ERR. | ||
M1 | 86,66 | 32,40 | 1,78 | 5,49 | 37,36 | 2,10 | 5,63 | 10,84 | 4,50 | 41,48 | 12,56 | 5,42 | 43,13 |
M2 | 64,93 | 47,80 | 5,01 | 10,49 | 53,41 | 4,16 | 7,79 | 32,36 | 6,06 | 18,74 | 35,57 | 9,43 | 26,52 |
M3 | 3,30 | 27,84 | 1,50 | 5,37 | 25,14 | 1,37 | 5,44 | 57,68 | 4,79 | 8,31 | 50,80 | 6,81 | 13,40 |
M4 | 96,59 | 51,06 | 4,14 | 8,10 | 57,78 | 3,82 | 6,61 | 28,87 | 7,62 | 26,40 | 32,36 | 10,33 | 31,93 |