Team:Warsaw/Calendar-Stage1/24 July 2010

From 2010.igem.org

Revision as of 18:29, 26 October 2010 by Czeriberi (Talk | contribs)

Example Tabs


23.07.2010 Friday

Previous 2 days: Set up and culturing Hela cell culture [Kamil]

Use a protocol...

Previous day: Set up liquid cultures of different types of E.coli [Ania P.]

Use a protocol...

Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]

1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells and examining them in flow cytometer

Data analysis with Cell Quest and Microsoft Excel [Cherry]


legend:

E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP

Hela cells with bacteria:
23-07-2010.007- without bacteria
23-07-2010.018- with G (1)
23-07-2010.019- with G (2)
23-07-2010.020- with G (3)
23-07-2010.015- with I (1)
23-07-2010.016- with I (2)
23-07-2010.017- with I (3)
23-07-2010.012- with J (1)
23-07-2010.013- with J (2)
23-07-2010.014- with J (3)
23-07-2010.008- with A (1)
23-07-2010.010- with A (2)
23-07-2010.011- with A (3)




Data processed with Cell Quest:







Data from Cell Quest processed with Microsoft Excel:



Hela g1 g2 g3 śr. odch. bląd i1 i2 i3 śr. odch. bląd
M1 86,66 32,19 34,27 30,73 32,39667 1,779026 5,491386 35,06 37,85 39,18 37,36333 2,102673 5,627637
M2 64,93 47,4 53 43 47,8 5,011986 10,48533 48,66 55,19 56,39 53,41333 4,160004 7,788325
M3 3,3 28,09 26,24 29,2 27,84333 1,495337 5,370539 26,45 25,26 23,72 25,14333 1,368734 5,443726
M4 96,59 50,63 55,4 47,16 51,06333 4,137056 8,101814 53,41 59,48 60,46 57,78333 3,818983 6,609143
                         
                         
  j1 j2 j3 śr. odch. bląd a1 a2 a3 śr. odch. bląd
  8,01 16,03 8,49 10,84333 4,498192 41,48348 18,78 10,07 8,84 12,56333 5,418804 43,13189
  28,51 39,35 29,22 32,36 6,063918 18,73893 46,45 29,68 30,58 35,57 9,433096 26,51981
  59,24 52,3 61,5 57,68 4,794288 8,311873 43,15 53,05 56,19 50,79667 6,805772 13,39807
  24,15 37,66 24,79 28,86667 7,62197 26,40405 44,29 26,48 26,31 32,36 10,33203 31,92841
                         
                         
  Hela Hela G     Hela I     Hela J     Hela A    
    śr. odch. bląd śr. odch. bląd śr. odch. bląd śr. odch. bląd
M1 86,66 32,39667 1,779026 5,491386 37,36333 2,102673 5,627637 10,84333 4,498192 41,48348 12,56333 5,418804 43,13189
M2 64,93 47,8 5,011986 10,48533 53,41333 4,160004 7,788325 32,36 6,063918 18,73893 35,57 9,433096 26,51981
M3 3,3 27,84333 1,495337 5,370539 25,14333 1,368734 5,443726 57,68 4,794288 8,311873 50,79667 6,805772 13,39807
M4 96,59 51,06333 4,137056 8,101814 57,78333 3,818983 6,609143 28,86667 7,62197 26,40405 32,36 10,33203 31,92841



Hela
M186,66
M264,93
M33,3
M496,59
g1g2g3
32,1934,2730,73
47,45343
28,0926,2429,2
50,6355,447,16
śr.SDERR.
32,41,785,49
47,85,0110,49
27,841,55,37
51,064,148,1
i1i2i3
35,0637,8539,18
48,6655,1956,39
26,4525,2623,72
53,4159,4860,46
śr.SDERR.
37,362,15,63
53,414,167,79
25,141,375,44
57,783,826,61


j1j2j3
8,0116,038,49
28,5139,3529,22
59,2452,361,5
24,1537,6624,79
śr.SDERR.
10,844,541,48
32,366,0618,74
57,684,798,31
28,877,6226,4
a1a2a3
18,7810,078,84
46,4529,6830,58
43,1553,0556,19
44,2926,4826,31
śr.SDERR.
12,565,4243,13
35,579,4326,52
50,86,8113,4
32,3610,3331,93