Team:Warsaw/Calendar-Stage1/20 September 2010


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Latest revision as of 20:24, 27 October 2010

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Cloning MinC under RBS and a promoter


pSB1A2 plasmids containig T7 promoter and B0032/B0034 RBS have been obtained from Michał. They were subsequently digested with SpeI and PstI. MinC-harboring plasmid was cut with XbaI and PstI. Following a 1,5 h digest, pSB1A2-pT7-B0032/34 and MinC were purified by gel electrophoresis with Gel-out kit (A&A biotechnology). Ligation was preformed over-night, with T4 DNA ligase (Fermentas) in 18 oC and standard reaction buffer