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Team:Warsaw/Calendar-Stage1/1 July 2010
From 2010.igem.org
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| + | <br> 1.07.2010 Thursday | ||
<br><div class="note"> Previous day: Preparation of competent cells [Ania P., Jarek]</div> | <br><div class="note"> Previous day: Preparation of competent cells [Ania P., Jarek]</div> | ||
<br> Use a protocol for competent cells preparation to get competent ''Escherichia coli'' TOP10 strain. | <br> Use a protocol for competent cells preparation to get competent ''Escherichia coli'' TOP10 strain. | ||
Revision as of 13:16, 31 July 2010
1.07.2010 Thursday
Previous day: Preparation of competent cells [Ania P., Jarek]
Use a protocol for competent cells preparation to get competent ''Escherichia coli'' TOP10 strain.
Previous day: Revitalisation of biobricks [Ania P., Jarek]
Biobricks from 2010 distribution: GFP-terminator, J61100 J61101 J61107 J61117 J61127 [Anderson's RBSes], B0030 B0031 B0032 B0033 B0034 [community RBSes], J23100 [synthetic promoter].
1. Distributed in 10 ul of TE [skład].
2. 5 ul of DNA used for transformation [according to protocol].
Checking correctness of biobricks [Ania P., Jarek]
1. Alkaline lysis no1 [according to protocol].
2. Restriction digest
3. Run agarose gel
