Team:Warsaw/Calendar-Stage1/1 July 2010
From 2010.igem.org
(Difference between revisions)
Line 15: | Line 15: | ||
<!-- div class note gives the strip with the light brown gackground, it is declared in the css for our webpage! --> | <!-- div class note gives the strip with the light brown gackground, it is declared in the css for our webpage! --> | ||
- | <div class="note"> | + | <br><div class="note"> Previous day: Preparation of competent cells [Ania P., Jarek]</div> |
+ | <br> Use a protocol for competent cells preparation to get competent ''Escherichia coli'' TOP10 strain. | ||
+ | <br> | ||
- | <br> | + | <br><div class="note"> Previous day: Revitalisation of biobricks [Ania P., Jarek]</div> |
- | <a href="http://partsregistry.org/Part:BBa_J61100"> | + | <br> Biobricks from 2010 distribution: |
- | [ | + | GFP-terminator, |
- | <br>2. | + | <a href="http://partsregistry.org/Part:BBa_J61100">J61100</a> |
+ | <a href="http://partsregistry.org/Part:BBa_J61101">J61101</a> | ||
+ | <a href="http://partsregistry.org/Part:BBa_J61107">J61107</a> | ||
+ | <a href="http://partsregistry.org/Part:BBa_J61117">J61117</a> | ||
+ | <a href="http://partsregistry.org/Part:BBa_J61127">J61127</a> | ||
+ | [Anderson's RBSes], | ||
+ | <a href="http://partsregistry.org/Part:BBa_B0030">B0030</a> | ||
+ | <a href="http://partsregistry.org/Part:BBa_B0031">B0031</a> | ||
+ | <a href="http://partsregistry.org/Part:BBa_B0032">B0032</a> | ||
+ | <a href="http://partsregistry.org/Part:BBa_B0033">B0033</a> | ||
+ | <a href="http://partsregistry.org/Part:BBa_B0034">B0034</a> | ||
+ | [community RBSes], | ||
+ | <a href="http://partsregistry.org/Part:BBa_J23100">J23100</a> | ||
+ | [synthetic promoter]. | ||
+ | <br> 1. Distributed in 10 ul of TE [skład]. | ||
+ | <br> 2. 5 ul of DNA used for transformation [according to protocol]. | ||
+ | <br> | ||
+ | <br><div class="note"> Checking correctness of biobricks [Ania P., Jarek]</div> | ||
+ | <br> 1. Alkaline lysis no1 [according to protocol]. | ||
+ | <br> 2. Restriction digest | ||
+ | <br> 3. Run agarose gel | ||
+ | <br> | ||
<!-- use br to go to the next line also to ctrate empty lines! --> | <!-- use br to go to the next line also to ctrate empty lines! --> | ||
Revision as of 20:10, 21 July 2010
Previous day: Preparation of competent cells [Ania P., Jarek]
Use a protocol for competent cells preparation to get competent ''Escherichia coli'' TOP10 strain.
Previous day: Revitalisation of biobricks [Ania P., Jarek]
Biobricks from 2010 distribution: GFP-terminator, J61100 J61101 J61107 J61117 J61127 [Anderson's RBSes], B0030 B0031 B0032 B0033 B0034 [community RBSes], J23100 [synthetic promoter].
1. Distributed in 10 ul of TE [skład].
2. 5 ul of DNA used for transformation [according to protocol].
Checking correctness of biobricks [Ania P., Jarek]
1. Alkaline lysis no1 [according to protocol].
2. Restriction digest
3. Run agarose gel